Identification of the protein encoded by the human diffuse B-cell lymphoma (dbl) oncogene

S. K. Srivastava, R. H P Wheelock, S. A. Aaronson, A. Eva

Research output: Contribution to journalArticle

Abstract

The dbl oncogene was initially isolated from a human diffuse B-cell lymphoma. Antisera from mice bearing tumors induced by this oncogene specifically detected a protein of about 66 kDa (p66) in dbl transformants. dbl cDNA-selected poly(A)+ RNA isolated from a transfectant clone expressing p66 directed the in vitro synthesis of this protein, establishing that it is encoded by dbl. Subcellular localization studies revealed that p66 is a cytoplasmic protein distributed between cytosol and crude membrane fractions. Moreover, p66 was shown to be a phosphoprotein, with phosphorylation specific to serine residues. Our characterization of the dbl-encoded protein appears to distinguish this transforming gene product from those of other known oncogenes.

Original languageEnglish
Pages (from-to)8868-8872
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number23
Publication statusPublished - 1986

ASJC Scopus subject areas

  • General
  • Genetics

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