Identification of the protein encoded by the human diffuse B-cell lymphoma (dbl) oncogene

S. K. Srivastava, R. H P Wheelock, S. A. Aaronson, A. Eva

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The dbl oncogene was initially isolated from a human diffuse B-cell lymphoma. Antisera from mice bearing tumors induced by this oncogene specifically detected a protein of about 66 kDa (p66) in dbl transformants. dbl cDNA-selected poly(A)+ RNA isolated from a transfectant clone expressing p66 directed the in vitro synthesis of this protein, establishing that it is encoded by dbl. Subcellular localization studies revealed that p66 is a cytoplasmic protein distributed between cytosol and crude membrane fractions. Moreover, p66 was shown to be a phosphoprotein, with phosphorylation specific to serine residues. Our characterization of the dbl-encoded protein appears to distinguish this transforming gene product from those of other known oncogenes.

Original languageEnglish
Pages (from-to)8868-8872
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number23
Publication statusPublished - 1986

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B-Cell Lymphoma
Oncogenes
Non-Hodgkin's Lymphoma
Proteins
Phosphoproteins
Cytosol
Serine
Immune Sera
Complementary DNA
Clone Cells
Phosphorylation
Messenger RNA
Membranes
Neoplasms

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Identification of the protein encoded by the human diffuse B-cell lymphoma (dbl) oncogene. / Srivastava, S. K.; Wheelock, R. H P; Aaronson, S. A.; Eva, A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 23, 1986, p. 8868-8872.

Research output: Contribution to journalArticle

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