Identification of tumor-associated antigens by screening phage-displayed human cDNA libraries with sera from tumor patients

Olga Minenkova, Andrea Pucci, Emiliano Pavoni, Amedeo De Tomassi, Paola Fortugno, Nicola Gargano, Maurizio Cianfriglia, Stefano Barca, Sabino De Placido, Angelo Martignetti, Franco Felici, Riccardo Cortese, Paolo Monaci

Research output: Contribution to journalArticle

Abstract

Screening cDNA libraries from solid human tumors with sera of autologous patients (SEREX) has proven to be a powerful approach to identifying tumor antigens recognized by the humoral arm of the immune system. In many cases, application of this methodology has led to the discovery of novel tumor antigens as unknown gene products. We tried to improve the potency of the SEREX approach by combining it with phage-display technology. We designed a new lambda vector to express protein fragments as N-terminal fusions to the D capsid protein and generated high-complexity cDNA libraries from human breast carcinoma cell lines and solid tumors. Screening these phage-displayed libraries required limited amounts of sera from patients and efficiently identified several tumor antigens specifically reacting with sera from breast cancer patients.

Original languageEnglish
Pages (from-to)534-544
Number of pages11
JournalInternational Journal of Cancer
Volume106
Issue number4
DOIs
Publication statusPublished - Sep 10 2003

Keywords

  • Breast cancer
  • Immune response
  • Phage display

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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    Minenkova, O., Pucci, A., Pavoni, E., De Tomassi, A., Fortugno, P., Gargano, N., Cianfriglia, M., Barca, S., De Placido, S., Martignetti, A., Felici, F., Cortese, R., & Monaci, P. (2003). Identification of tumor-associated antigens by screening phage-displayed human cDNA libraries with sera from tumor patients. International Journal of Cancer, 106(4), 534-544. https://doi.org/10.1002/ijc.11269