IDLV-HIV-1 Env vaccination in non-human primates induces affinity maturation of antigen-specific memory B cells

Maria Blasi, Donatella Negri, Celia LaBranche, S Munir Alam, Erich J Baker, Elizabeth C Brunner, Morgan A Gladden, Zuleika Michelini, Nathan A Vandergrift, Kevin J Wiehe, Robert Parks, Xiaoying Shen, Mattia Bonsignori, Georgia D Tomaras, Guido Ferrari, David C Montefiori, Sampa Santra, Barton F Haynes, Michael A Moody, Andrea CaraMary E Klotman

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Abstract

HIV continues to be a major global health issue. In spite of successful prevention interventions and treatment methods, the development of an HIV vaccine remains a major priority for the field and would be the optimal strategy to prevent new infections. We showed previously that a single immunization with a SIV-based integrase-defective lentiviral vector (IDLV) expressing the 1086.C HIV-1-envelope induced durable, high-magnitude immune responses in non-human primates (NHPs). In this study, we have further characterized the humoral responses by assessing antibody affinity maturation and antigen-specific memory B-cell persistence in two vaccinated macaques. These animals were also boosted with IDLV expressing the heterologous 1176.C HIV-1-Env to determine if neutralization breadth could be increased, followed by evaluation of the injection sites to assess IDLV persistence. IDLV-Env immunization was associated with persistence of the vector DNA for up to 6 months post immunization and affinity maturation of antigen-specific memory B cells.

Original languageEnglish
Pages (from-to)134
JournalCommunications biology
Volume1
DOIs
Publication statusPublished - 2018

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Keywords

  • HIV-1 infection
  • Vaccination
  • Non-human primates
  • B cell memory

Cite this

Blasi, M., Negri, D., LaBranche, C., Alam, S. M., Baker, E. J., Brunner, E. C., Gladden, M. A., Michelini, Z., Vandergrift, N. A., Wiehe, K. J., Parks, R., Shen, X., Bonsignori, M., Tomaras, G. D., Ferrari, G., Montefiori, D. C., Santra, S., Haynes, B. F., Moody, M. A., ... Klotman, M. E. (2018). IDLV-HIV-1 Env vaccination in non-human primates induces affinity maturation of antigen-specific memory B cells. Communications biology, 1, 134. https://doi.org/10.1038/s42003-018-0131-6