IDLV-HIV-1 Env vaccination in non-human primates induces affinity maturation of antigen-specific memory B cells

Maria Blasi, Donatella Negri, Celia LaBranche, S Munir Alam, Erich J Baker, Elizabeth C Brunner, Morgan A Gladden, Zuleika Michelini, Nathan A Vandergrift, Kevin J Wiehe, Robert Parks, Xiaoying Shen, Mattia Bonsignori, Georgia D Tomaras, Guido Ferrari, David C Montefiori, Sampa Santra, Barton F Haynes, Michael A Moody, Andrea CaraMary E Klotman

Research output: Contribution to journalArticlepeer-review

Abstract

HIV continues to be a major global health issue. In spite of successful prevention interventions and treatment methods, the development of an HIV vaccine remains a major priority for the field and would be the optimal strategy to prevent new infections. We showed previously that a single immunization with a SIV-based integrase-defective lentiviral vector (IDLV) expressing the 1086.C HIV-1-envelope induced durable, high-magnitude immune responses in non-human primates (NHPs). In this study, we have further characterized the humoral responses by assessing antibody affinity maturation and antigen-specific memory B-cell persistence in two vaccinated macaques. These animals were also boosted with IDLV expressing the heterologous 1176.C HIV-1-Env to determine if neutralization breadth could be increased, followed by evaluation of the injection sites to assess IDLV persistence. IDLV-Env immunization was associated with persistence of the vector DNA for up to 6 months post immunization and affinity maturation of antigen-specific memory B cells.

Original languageEnglish
Pages (from-to)134
JournalCommunications Biology
Volume1
DOIs
Publication statusPublished - 2018

Keywords

  • HIV-1 infection
  • Vaccination
  • Non-human primates
  • B cell memory

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