TY - JOUR
T1 - IFI16 expression is related to selected transcription factors during B-cell differentiation
AU - Piccaluga, Pier Paolo
AU - Agostinelli, Claudio
AU - Fuligni, Fabio
AU - Righi, Simona
AU - Tripodo, Claudio
AU - Re, Maria Carla
AU - Clò, Alberto
AU - Miserocchi, Anna
AU - Morini, Silvia
AU - Gariglio, Marisa
AU - Ferri, Gian Gaetano
AU - Rinaldi-Ceroni, Alberto
AU - Piccin, Ottavio
AU - De Andrea, Marco
AU - Pileri, Stefano A.
AU - Landolfo, Santo
AU - Gibellini, Davide
PY - 2015
Y1 - 2015
N2 - The interferon-inducible DNA sensor IFI16 is involved in the modulation of cellular survival, proliferation, and differentiation. In the hematopoietic system, IFI16 is consistently expressed in the CD34+ stem cells and in peripheral blood lymphocytes; however, little is known regarding its regulation during maturation of B- and T-cells. We explored the role of IFI16 in normal B-cell subsets by analysing its expression and relationship with the major transcription factors involved in germinal center (GC) development and plasma-cell (PC) maturation. IFI16 mRNA was differentially expressed in B-cell subsets with significant decrease in IFI16 mRNA in GC and PCs with respect to naïve and memory subsets. IFI16 mRNA expression is inversely correlated with a few master regulators of B-cell differentiation such as BCL6, XBP1, POU2AF1, and BLIMP1. In contrast, IFI16 expression positively correlated with STAT3, REL, SPIB, RELA, RELB, IRF4, STAT5B, and STAT5A. ARACNE algorithm indicated a direct regulation of IFI16 by BCL6, STAT5B, and RELB, whereas the relationship between IFI16 and the other factors is modulated by intermediate factors. In addition, analysis of the CD40 signaling pathway showed that IFI16 gene expression directly correlated with NF-B activation, indicating that IFI16 could be considered an upstream modulator of NF-B in human B-cells.
AB - The interferon-inducible DNA sensor IFI16 is involved in the modulation of cellular survival, proliferation, and differentiation. In the hematopoietic system, IFI16 is consistently expressed in the CD34+ stem cells and in peripheral blood lymphocytes; however, little is known regarding its regulation during maturation of B- and T-cells. We explored the role of IFI16 in normal B-cell subsets by analysing its expression and relationship with the major transcription factors involved in germinal center (GC) development and plasma-cell (PC) maturation. IFI16 mRNA was differentially expressed in B-cell subsets with significant decrease in IFI16 mRNA in GC and PCs with respect to naïve and memory subsets. IFI16 mRNA expression is inversely correlated with a few master regulators of B-cell differentiation such as BCL6, XBP1, POU2AF1, and BLIMP1. In contrast, IFI16 expression positively correlated with STAT3, REL, SPIB, RELA, RELB, IRF4, STAT5B, and STAT5A. ARACNE algorithm indicated a direct regulation of IFI16 by BCL6, STAT5B, and RELB, whereas the relationship between IFI16 and the other factors is modulated by intermediate factors. In addition, analysis of the CD40 signaling pathway showed that IFI16 gene expression directly correlated with NF-B activation, indicating that IFI16 could be considered an upstream modulator of NF-B in human B-cells.
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U2 - 10.1155/2015/747645
DO - 10.1155/2015/747645
M3 - Article
AN - SCOPUS:84936817449
VL - 2015
JO - Journal of Immunology Research
JF - Journal of Immunology Research
SN - 2314-8861
M1 - 747645
ER -