IFN-γ inhibits the proliferation of allergen-activated T lymphocytes from atopic, asthmatic patients by inducing Fas/FasL-mediated apoptosis

Virginia De Rose, Paola Cappello, Valentina Sorbello, Barbara Ceccarini, Federica Gani, Marita Bosticardo, Stefania Fassio, Francesco Novelli

Research output: Contribution to journalArticle

Abstract

The defect in interferon-γ (IFN-γ) production that results in a T helper cell type 2-dominated response may be responsible for a decrease in the apoptosis of allergen-activated T cells in asthma. We investigated the effect of recombinant IFN-γ on proliferation, Fas/Fas ligand (FasL) expression, and apoptosis in allergen-stimulated peripheral blood mononuclear cells obtained from atopic, asthmatic patients and nonatopic, control subjects. The addition of IFN-γ at the start of cultures markedly inhibited the proliferative response to a specific allergen in cells from all asthmatic patients, whereas no change was observed in cells from nonatopic, control subjects. IFN-γ induced an increase in the expression of Fas and FasL by allergen-stimulated CD4+ T cells from asthmatic patients and caused the apoptosis of these cells. A Fas-blocking monoclonal antibody prevented the inhibitory effect of IFN-γ on allergen-induced proliferation. These results suggest that IFN-γ inhibits the proliferation of allergen-stimulated CD4+ T cells from atopic, asthmatic patients by inducing the surface expression of Fas and FasL, which in turn triggers their apoptotic program. The defect in IFN-γ production involved in the allergic, immune response may therefore be responsible for a decrease in apoptosis of allergen-activated T lymphocytes in the airways of atopic, asthmatic patients.

Original languageEnglish
Pages (from-to)423-432
Number of pages10
JournalJournal of Leukocyte Biology
Volume76
Issue number2
DOIs
Publication statusPublished - Aug 2004

Keywords

  • Allergy
  • Asthma
  • Cytokines
  • Inflammation

ASJC Scopus subject areas

  • Cell Biology

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    De Rose, V., Cappello, P., Sorbello, V., Ceccarini, B., Gani, F., Bosticardo, M., Fassio, S., & Novelli, F. (2004). IFN-γ inhibits the proliferation of allergen-activated T lymphocytes from atopic, asthmatic patients by inducing Fas/FasL-mediated apoptosis. Journal of Leukocyte Biology, 76(2), 423-432. https://doi.org/10.1189/jlb.0503247