IFN-γ inhibits the proliferation of allergen-activated T lymphocytes from atopic, asthmatic patients by inducing Fas/FasL-mediated apoptosis

Virginia De Rose, Paola Cappello, Valentina Sorbello, Barbara Ceccarini, Federica Gani, Marita Bosticardo, Stefania Fassio, Francesco Novelli

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The defect in interferon-γ (IFN-γ) production that results in a T helper cell type 2-dominated response may be responsible for a decrease in the apoptosis of allergen-activated T cells in asthma. We investigated the effect of recombinant IFN-γ on proliferation, Fas/Fas ligand (FasL) expression, and apoptosis in allergen-stimulated peripheral blood mononuclear cells obtained from atopic, asthmatic patients and nonatopic, control subjects. The addition of IFN-γ at the start of cultures markedly inhibited the proliferative response to a specific allergen in cells from all asthmatic patients, whereas no change was observed in cells from nonatopic, control subjects. IFN-γ induced an increase in the expression of Fas and FasL by allergen-stimulated CD4+ T cells from asthmatic patients and caused the apoptosis of these cells. A Fas-blocking monoclonal antibody prevented the inhibitory effect of IFN-γ on allergen-induced proliferation. These results suggest that IFN-γ inhibits the proliferation of allergen-stimulated CD4+ T cells from atopic, asthmatic patients by inducing the surface expression of Fas and FasL, which in turn triggers their apoptotic program. The defect in IFN-γ production involved in the allergic, immune response may therefore be responsible for a decrease in apoptosis of allergen-activated T lymphocytes in the airways of atopic, asthmatic patients.

Original languageEnglish
Pages (from-to)423-432
Number of pages10
JournalJournal of Leukocyte Biology
Volume76
Issue number2
DOIs
Publication statusPublished - Aug 2004

Fingerprint

Fas Ligand Protein
Allergens
Interferons
Apoptosis
T-Lymphocytes
Th2 Cells
Blocking Antibodies
Blood Cells
Asthma
Monoclonal Antibodies

Keywords

  • Allergy
  • Asthma
  • Cytokines
  • Inflammation

ASJC Scopus subject areas

  • Cell Biology

Cite this

IFN-γ inhibits the proliferation of allergen-activated T lymphocytes from atopic, asthmatic patients by inducing Fas/FasL-mediated apoptosis. / De Rose, Virginia; Cappello, Paola; Sorbello, Valentina; Ceccarini, Barbara; Gani, Federica; Bosticardo, Marita; Fassio, Stefania; Novelli, Francesco.

In: Journal of Leukocyte Biology, Vol. 76, No. 2, 08.2004, p. 423-432.

Research output: Contribution to journalArticle

De Rose, V, Cappello, P, Sorbello, V, Ceccarini, B, Gani, F, Bosticardo, M, Fassio, S & Novelli, F 2004, 'IFN-γ inhibits the proliferation of allergen-activated T lymphocytes from atopic, asthmatic patients by inducing Fas/FasL-mediated apoptosis', Journal of Leukocyte Biology, vol. 76, no. 2, pp. 423-432. https://doi.org/10.1189/jlb.0503247
De Rose, Virginia ; Cappello, Paola ; Sorbello, Valentina ; Ceccarini, Barbara ; Gani, Federica ; Bosticardo, Marita ; Fassio, Stefania ; Novelli, Francesco. / IFN-γ inhibits the proliferation of allergen-activated T lymphocytes from atopic, asthmatic patients by inducing Fas/FasL-mediated apoptosis. In: Journal of Leukocyte Biology. 2004 ; Vol. 76, No. 2. pp. 423-432.
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