TY - JOUR
T1 - IFN-γ low production capacity in type 1 diabetes mellitus patients at onset of disease
AU - Avanzini, Maria A.
AU - Ciardelli, L.
AU - Lenta, E.
AU - Castellazzi, A. M.
AU - Marconi, M.
AU - Derosa, G.
AU - Dos Santos, C.
AU - Oliveri, M.
AU - Pistorio, A.
AU - Lorini, R.
AU - D'Annunzio, G.
PY - 2005/6
Y1 - 2005/6
N2 - In type 1 diabetes mellitus (T1DM), cytokines can be directly cytotoxic to β-cells, and/or play an indirect role influencing some cells of the immune system. Since several factors could impair cytokine serum levels, the purpose of our study was to longitudi-nally evaluate intracellular cytokines, in T1DM patients, and in subject at risk, by flow cytometry analysis. At T1DM onset we observed significantly lower percentage of peripheral CD4+ and CD8+ cells producing IFN-γ in patients compared to controls and subjects at risk. The 15-month follow-up patients showed significantly lower percentage of CD4+ and CD8+ cells producing IFN-γ compared to the other groups. At 8-year follow-up no significant differences were observed among the groups in the percentage of cells producing cytokines. We could have considered "exhausted cells" or these T cell subsets may be migrated from peripheral blood to pancreas. On the other hand, our results are in agreement with those reported in literature: in animal model the absence of IFN-γ production makes β-cells highly susceptible to viral infection and subsequent attack by natural killer cells, which lead to hyperglycaemia and diabetes mellitus.
AB - In type 1 diabetes mellitus (T1DM), cytokines can be directly cytotoxic to β-cells, and/or play an indirect role influencing some cells of the immune system. Since several factors could impair cytokine serum levels, the purpose of our study was to longitudi-nally evaluate intracellular cytokines, in T1DM patients, and in subject at risk, by flow cytometry analysis. At T1DM onset we observed significantly lower percentage of peripheral CD4+ and CD8+ cells producing IFN-γ in patients compared to controls and subjects at risk. The 15-month follow-up patients showed significantly lower percentage of CD4+ and CD8+ cells producing IFN-γ compared to the other groups. At 8-year follow-up no significant differences were observed among the groups in the percentage of cells producing cytokines. We could have considered "exhausted cells" or these T cell subsets may be migrated from peripheral blood to pancreas. On the other hand, our results are in agreement with those reported in literature: in animal model the absence of IFN-γ production makes β-cells highly susceptible to viral infection and subsequent attack by natural killer cells, which lead to hyperglycaemia and diabetes mellitus.
KW - CD4+
KW - CD8+
KW - Cytokines
KW - IFN-γ
KW - T1DM
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U2 - 10.1055/s-2005-865643
DO - 10.1055/s-2005-865643
M3 - Article
C2 - 15977097
AN - SCOPUS:21244490100
VL - 113
SP - 313
EP - 317
JO - Experimental and Clinical Endocrinology and Diabetes
JF - Experimental and Clinical Endocrinology and Diabetes
SN - 0947-7349
IS - 6
ER -