IGF-I induced rapid recruitment of integrin β1 to lipid rafts is Caveolin-1 dependent

B. Salani, L. Briatore, P. Contini, M. Passalacqua, E. Melloni, A. Paggi, R. Cordera, D. Maggi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Caveolin-1 (Cav-1) regulates both insulin like growth factor receptor (IGF-IR) and integrin β1 function. However, the role of Cav-1 in IGF-IR/integrin β1 cross talk remains to be established. In this study, we observed that IGF-I did not induce integrin β1 internalization but its plasma membrane reorganization. In particular, we found a rapid and transient association between integrin β1 and Cav-1 followed by the enrichment of integrin β1 in lipid rafts. To determine the role of Cav-1 in this process, we transfected Hacat cells with small interfering RNA specific for Cav-1 (siRNA-Cav-1) and with a scrambled siRNA as control (siRNA-Ctr). Cav-1 down regulated Hacat cells were then stimulated with IGF-I and analyzed by immunofluorescence and flow cytometry. We found that Cav-1 silencing abolished the recruitment of integrin β1 to lipid rafts in the presence of IGF-I. These data demonstrate that IGF-IR/integrin β1 cross talk is followed by integrin β1 lipid raft compartmentalization and that Cav-1 is required for this process.

Original languageEnglish
Pages (from-to)489-492
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume380
Issue number3
DOIs
Publication statusPublished - Mar 13 2009

Fingerprint

Caveolin 1
Insulin-Like Growth Factor I
Integrins
Lipids
Small Interfering RNA
Somatomedin Receptors
Flow cytometry
Cell membranes
Fluorescent Antibody Technique
Flow Cytometry
Cell Membrane

Keywords

  • Caveolin
  • IGF-IR
  • Integrin
  • Lipid rafts

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Salani, B., Briatore, L., Contini, P., Passalacqua, M., Melloni, E., Paggi, A., ... Maggi, D. (2009). IGF-I induced rapid recruitment of integrin β1 to lipid rafts is Caveolin-1 dependent. Biochemical and Biophysical Research Communications, 380(3), 489-492. https://doi.org/10.1016/j.bbrc.2009.01.102

IGF-I induced rapid recruitment of integrin β1 to lipid rafts is Caveolin-1 dependent. / Salani, B.; Briatore, L.; Contini, P.; Passalacqua, M.; Melloni, E.; Paggi, A.; Cordera, R.; Maggi, D.

In: Biochemical and Biophysical Research Communications, Vol. 380, No. 3, 13.03.2009, p. 489-492.

Research output: Contribution to journalArticle

Salani, B, Briatore, L, Contini, P, Passalacqua, M, Melloni, E, Paggi, A, Cordera, R & Maggi, D 2009, 'IGF-I induced rapid recruitment of integrin β1 to lipid rafts is Caveolin-1 dependent', Biochemical and Biophysical Research Communications, vol. 380, no. 3, pp. 489-492. https://doi.org/10.1016/j.bbrc.2009.01.102
Salani, B. ; Briatore, L. ; Contini, P. ; Passalacqua, M. ; Melloni, E. ; Paggi, A. ; Cordera, R. ; Maggi, D. / IGF-I induced rapid recruitment of integrin β1 to lipid rafts is Caveolin-1 dependent. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 380, No. 3. pp. 489-492.
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