IGF-I induces caveolin 1 tyrosine phosphorylation and translocation in the lipid rafts

Davide Maggi, Claudia Biedi, Daniela Segat, Daniela Barbero, Danilo Panetta, Renzo Cordera

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Caveolin 1, a component of caveolae, regulates signalling pathways compartmentalization interacting with tyrosine kinase receptors and their substrates. The role of caveolin 1 in the Insulin Receptor (IR) signalling has been well investigated. On the contrary, the functional link between caveolin 1 and IGF-I Receptor (IGF-IR) remains largely unknown. Here we show that (1) IGF-IR colocalizes with caveolin 1 in the lipid rafts enriched fractions on plasmamembrane in R-IGF-IRWT cells, (2) IGF-I induces caveolin 1 phosphorylation at the level of tyrosine 14, (3) this effect is rapid and results in the translocation of caveolin 1 and in the formation of membrane patches on cell surface. These actions are IGF-I specific since we did not detect caveolin 1 redistribution in insulin stimulated R- cells overexpressing IRs.

Original languageEnglish
Pages (from-to)1085-1089
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume295
Issue number5
DOIs
Publication statusPublished - 2002

Fingerprint

Caveolin 1
Phosphorylation
Insulin-Like Growth Factor I
Tyrosine
Lipids
IGF Type 1 Receptor
Caveolae
Insulin Receptor
Receptor Protein-Tyrosine Kinases
Insulin
Membranes
Substrates

Keywords

  • Caveolin 1
  • IGF-I receptor
  • Insulin receptor
  • Lipid raft
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

IGF-I induces caveolin 1 tyrosine phosphorylation and translocation in the lipid rafts. / Maggi, Davide; Biedi, Claudia; Segat, Daniela; Barbero, Daniela; Panetta, Danilo; Cordera, Renzo.

In: Biochemical and Biophysical Research Communications, Vol. 295, No. 5, 2002, p. 1085-1089.

Research output: Contribution to journalArticle

Maggi, Davide ; Biedi, Claudia ; Segat, Daniela ; Barbero, Daniela ; Panetta, Danilo ; Cordera, Renzo. / IGF-I induces caveolin 1 tyrosine phosphorylation and translocation in the lipid rafts. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 295, No. 5. pp. 1085-1089.
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