TY - JOUR
T1 - IGF-I regulates caveolin 1 and IRS1 interaction in caveolae
AU - Panetta, Danilo
AU - Biedi, Claudia
AU - Repetto, Silvia
AU - Cordera, Renzo
AU - Maggi, Davide
PY - 2004/3/26
Y1 - 2004/3/26
N2 - Caveolae are hot spots in IGF-I signalling as suggested by the facts that IGF-I receptors localize in caveolae, directly interact with and tyrosine phosphorylate caveolin 1, the major caveolar protein. Also a number of IGF-IR substrates reside in caveolae, supporting a role of these organelles in the regulation of IGF-I action. Recently, we have demonstrated that IGF-I could specifically regulate Shc phosphorylation in caveolae. Here we show that also IRS1 localizes in this region where it is tyrosine phosphorylated in the presence of IGF-I. Moreover, IRS1 co-immunoprecipitates with caveolin 1 and the specific phosphocaveolin 1-IRS1 interaction is increased by IGF-I.
AB - Caveolae are hot spots in IGF-I signalling as suggested by the facts that IGF-I receptors localize in caveolae, directly interact with and tyrosine phosphorylate caveolin 1, the major caveolar protein. Also a number of IGF-IR substrates reside in caveolae, supporting a role of these organelles in the regulation of IGF-I action. Recently, we have demonstrated that IGF-I could specifically regulate Shc phosphorylation in caveolae. Here we show that also IRS1 localizes in this region where it is tyrosine phosphorylated in the presence of IGF-I. Moreover, IRS1 co-immunoprecipitates with caveolin 1 and the specific phosphocaveolin 1-IRS1 interaction is increased by IGF-I.
KW - Caveolin 1
KW - IGF-I receptor
KW - IRS1
KW - Tyrosine phosphorylation
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U2 - 10.1016/j.bbrc.2004.02.037
DO - 10.1016/j.bbrc.2004.02.037
M3 - Article
C2 - 15003536
AN - SCOPUS:1542315620
VL - 316
SP - 240
EP - 243
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -