IgG anti-pentraxin 3 antibodies in systemic lupus erythematosus

N. Bassi, A. Ghirardello, M. Blank, S. Zampieri, P. Sarzi-Puttini, A. Mantovani, Y. Shoenfeld, Andrea Doria

Research output: Contribution to journalArticle

Abstract

Objective: To evaluate the prevalence and correlates of anti-pentraxin 3 (PTX3) antibodies in systemic lupus erythematosus (SLE). Methods: Serum samples from 130 patients with SLE, 130 age- and sex-matched healthy subjects and 130 patients with other autoimmune rheumatic diseases (oARD) were analysed by home-made ELISAs using as substrate human recombinant PTX3 and two peptides, PTX3-1 and PTX3-2, obtained from the complete protein, identified as potential antigenic sites using the Lasergene DNA program (DNA Star). Inhibition tests were performed to evaluate potential interferences between bovine serum albumin or C-reactive protein and anti-PTX3 or anti-PTX3 peptides, and between antigens and antibodies. Statistical analysis was performed using receiving operating characteristics curves, the Fisher exact test, two-tailed t test and Pearson correlations. Results: Patients with SLE had higher levels and prevalence of anti-PTX3, anti-PTX3-1 and anti-PTX3-2 antibodies than patients with oARD or healthy controls (p

Original languageEnglish
Pages (from-to)1704-1710
Number of pages7
JournalAnnals of the Rheumatic Diseases
Volume69
Issue number9
DOIs
Publication statusPublished - Sep 2010

ASJC Scopus subject areas

  • Rheumatology
  • Immunology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Allergy

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    Bassi, N., Ghirardello, A., Blank, M., Zampieri, S., Sarzi-Puttini, P., Mantovani, A., Shoenfeld, Y., & Doria, A. (2010). IgG anti-pentraxin 3 antibodies in systemic lupus erythematosus. Annals of the Rheumatic Diseases, 69(9), 1704-1710. https://doi.org/10.1136/ard.2009.117804