TY - JOUR
T1 - IgG anti-pentraxin 3 antibodies in systemic lupus erythematosus
AU - Bassi, N.
AU - Ghirardello, A.
AU - Blank, M.
AU - Zampieri, S.
AU - Sarzi-Puttini, P.
AU - Mantovani, A.
AU - Shoenfeld, Y.
AU - Doria, Andrea
PY - 2010/9
Y1 - 2010/9
N2 - Objective: To evaluate the prevalence and correlates of anti-pentraxin 3 (PTX3) antibodies in systemic lupus erythematosus (SLE). Methods: Serum samples from 130 patients with SLE, 130 age- and sex-matched healthy subjects and 130 patients with other autoimmune rheumatic diseases (oARD) were analysed by home-made ELISAs using as substrate human recombinant PTX3 and two peptides, PTX3-1 and PTX3-2, obtained from the complete protein, identified as potential antigenic sites using the Lasergene DNA program (DNA Star). Inhibition tests were performed to evaluate potential interferences between bovine serum albumin or C-reactive protein and anti-PTX3 or anti-PTX3 peptides, and between antigens and antibodies. Statistical analysis was performed using receiving operating characteristics curves, the Fisher exact test, two-tailed t test and Pearson correlations. Results: Patients with SLE had higher levels and prevalence of anti-PTX3, anti-PTX3-1 and anti-PTX3-2 antibodies than patients with oARD or healthy controls (p
AB - Objective: To evaluate the prevalence and correlates of anti-pentraxin 3 (PTX3) antibodies in systemic lupus erythematosus (SLE). Methods: Serum samples from 130 patients with SLE, 130 age- and sex-matched healthy subjects and 130 patients with other autoimmune rheumatic diseases (oARD) were analysed by home-made ELISAs using as substrate human recombinant PTX3 and two peptides, PTX3-1 and PTX3-2, obtained from the complete protein, identified as potential antigenic sites using the Lasergene DNA program (DNA Star). Inhibition tests were performed to evaluate potential interferences between bovine serum albumin or C-reactive protein and anti-PTX3 or anti-PTX3 peptides, and between antigens and antibodies. Statistical analysis was performed using receiving operating characteristics curves, the Fisher exact test, two-tailed t test and Pearson correlations. Results: Patients with SLE had higher levels and prevalence of anti-PTX3, anti-PTX3-1 and anti-PTX3-2 antibodies than patients with oARD or healthy controls (p
UR - http://www.scopus.com/inward/record.url?scp=77956025067&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77956025067&partnerID=8YFLogxK
U2 - 10.1136/ard.2009.117804
DO - 10.1136/ard.2009.117804
M3 - Article
C2 - 20439287
AN - SCOPUS:77956025067
VL - 69
SP - 1704
EP - 1710
JO - Annals of the Rheumatic Diseases
JF - Annals of the Rheumatic Diseases
SN - 0003-4967
IS - 9
ER -