IgG platelet antibodies in EDTA-dependent pseudothrombocytopenia bind to platelet membrane glycoprotein IIb

Francesco Fiorin, Agostino Steffan, Paola Pradella, Nicola Bizzaro, Rocco Potenza, Vincenzo De Angelis

Research output: Contribution to journalArticlepeer-review

Abstract

EDTA-dependent pseudothrombocytopenia (PTCP) consists of an inappropriate low platelet count caused by autoantibodies present in the serum samples reacting with platelets only in EDTA-anticoagulated blood. By using immunoprecipitation and Western blot techniques, we studied the immunochemical specificity of platelet agglutinating autoantibodies in the serum samples of 10 patients with PTCP. Furthermore, to evaluate a possible role of PTCP-associated IgG autoantibodies in increased platelet turnover, we assayed the plasma glycocalicin (GC) level and calculated the GC index for every patient. Our results provide direct evidence that an epitope located on platelet membrane glycoprotein IIb is recognized by PTCP-associated IgG antibodies; moreover, GC levels in patients with EDTA-dependent PTCP were similar to control levels, thus excluding an increased platelet turnover. We conclude that antiplatelet antibodies directed against platelet cryptantigens are unlikely to have a major role in the increased removal of cells from circulation.

Original languageEnglish
Pages (from-to)178-183
Number of pages6
JournalAmerican Journal of Clinical Pathology
Volume110
Issue number2
Publication statusPublished - Aug 1998

Keywords

  • Glycocalicin
  • Platelet antibodies
  • Platelet glycoprotein IIb
  • Pseudothrombocytopenia

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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