IgG subclass distribution of anti-HBs antibodies following vaccination with cDNA HBsAg

R. M. Borzì; P. Dal Monte; M. C. Honorati; A. Facchini

doi:10.1016/0022-1759(92)90043-S

IgG subclass distribution of anti-HBs antibodies following vaccination with cDNA HBsAg

R. M. Borzì, P. Dal Monte, M. C. Honorati, A. Facchini

Istituti Ortopedici Rizzoli

Research output: Contribution to journal › Article › peer-review

Abstract

Serum samples were obtained during follow-up of nine young adults vaccinated over 1 year with cDNA hepatitis B antigen. The absolute amounts of anti-HBs IgG subclass antibodies present in the sera were determined by comparing the optical densities (OD) obtained using an antigen-specific ELISA with those obtained by serial dilutions of known amounts of human IgG1-4. The calibration curves for each IgG subclass were corrected for the corresponding coating efficiency. Our data suggest that HBs antibody responses of vaccinated subjects occur in all IgG subclasses but IgG1 and IgG2 are the major subclasses involved.

Original language	English
Pages (from-to)	17-23
Number of pages	7
Journal	Journal of Immunological Methods
Volume	146
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(92)90043-S
Publication status	Published - 1992

Keywords

HBsAg
IgG subclass
Vaccination

ASJC Scopus subject areas

Biotechnology
Immunology

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title = "IgG subclass distribution of anti-HBs antibodies following vaccination with cDNA HBsAg",

abstract = "Serum samples were obtained during follow-up of nine young adults vaccinated over 1 year with cDNA hepatitis B antigen. The absolute amounts of anti-HBs IgG subclass antibodies present in the sera were determined by comparing the optical densities (OD) obtained using an antigen-specific ELISA with those obtained by serial dilutions of known amounts of human IgG1-4. The calibration curves for each IgG subclass were corrected for the corresponding coating efficiency. Our data suggest that HBs antibody responses of vaccinated subjects occur in all IgG subclasses but IgG1 and IgG2 are the major subclasses involved.",

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AU - Honorati, M. C.

AU - Facchini, A.

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AB - Serum samples were obtained during follow-up of nine young adults vaccinated over 1 year with cDNA hepatitis B antigen. The absolute amounts of anti-HBs IgG subclass antibodies present in the sera were determined by comparing the optical densities (OD) obtained using an antigen-specific ELISA with those obtained by serial dilutions of known amounts of human IgG1-4. The calibration curves for each IgG subclass were corrected for the corresponding coating efficiency. Our data suggest that HBs antibody responses of vaccinated subjects occur in all IgG subclasses but IgG1 and IgG2 are the major subclasses involved.

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