IgG subclass distribution of anti-HBs antibodies following vaccination with cDNA HBsAg

R. M. Borzì, P. Dal Monte, M. C. Honorati, A. Facchini

Research output: Contribution to journalArticlepeer-review

Abstract

Serum samples were obtained during follow-up of nine young adults vaccinated over 1 year with cDNA hepatitis B antigen. The absolute amounts of anti-HBs IgG subclass antibodies present in the sera were determined by comparing the optical densities (OD) obtained using an antigen-specific ELISA with those obtained by serial dilutions of known amounts of human IgG1-4. The calibration curves for each IgG subclass were corrected for the corresponding coating efficiency. Our data suggest that HBs antibody responses of vaccinated subjects occur in all IgG subclasses but IgG1 and IgG2 are the major subclasses involved.

Original languageEnglish
Pages (from-to)17-23
Number of pages7
JournalJournal of Immunological Methods
Volume146
Issue number1
DOIs
Publication statusPublished - 1992

Keywords

  • HBsAg
  • IgG subclass
  • Vaccination

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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