IgVH and bcl6 somatic mutation analysis reveals the heterogeneity of cutaneous B-cell lymphoma, and indicates the presence of undisclosed local antigens

Renato Franco, Francisca I. Camacho, Amalia Fernández-Vázquez, Patrocinio Algara, José L. Rodríguez-Peralto, Gaetano De Rosa, Miguel A. Piris

Research output: Contribution to journalArticle

Abstract

Our understanding of the ontology of B-cell lymphomas (BCL) has been improved by the study of mutational status of IgVH and bcl6 genes, but only a few cases of cutaneous BCL have been examined for this status. We analyzed IgVH and bcl6 somatic mutations in 10 cutaneous BCL, classified as follicular (three primary and one secondary), primary marginal zone (two cases), and diffuse large BCL (three primary and one secondary). We observed a lower rate (H mutation in all marginal zone lymphomas, and a preferential usage of VH2-70 (one primary follicular and two primary diffuse large BCL). Fewer than expected replacement mutations in framework regions (FR) were observed In three primary follicular lymphomas (FLs) and in all diffuse large BCL, indicating a negative antigen selection pressure. Ongoing mutations were observed in eight of 10 cases. Only two primary FLs and two diffuse large BCL showed bcl6 somatic mutation. These data support the heterogeneous nature of the different cutaneous BCL, and specifically the distinction between cutaneous follicular and marginal zone lymphomas. The biased usage of VH2-70, the low rate of replacement mutation in the FR, and the presence of ongoing mutation imply that local antigens could modulate the growth of primary cutaneous BCL.

Original languageEnglish
Pages (from-to)623-630
Number of pages8
JournalModern Pathology
Volume17
Issue number6
DOIs
Publication statusPublished - Jun 2004

Keywords

  • Cutaneous B-cell lymphoma
  • Somatic mutations

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Franco, R., Camacho, F. I., Fernández-Vázquez, A., Algara, P., Rodríguez-Peralto, J. L., De Rosa, G., & Piris, M. A. (2004). IgVH and bcl6 somatic mutation analysis reveals the heterogeneity of cutaneous B-cell lymphoma, and indicates the presence of undisclosed local antigens. Modern Pathology, 17(6), 623-630. https://doi.org/10.1038/modpathol.3800106