IL-1 inducible genes in human umbilical vein endothelial cells

M. Introna, F. Breviario, E. M. D'Aniello, J. Golay, E. Dejana, A. Mantovani

Research output: Contribution to journalArticle

Abstract

In an attempt to understand more directly the molecular mechanisms involved in the cellular response of endothelial cells to Interleukin 1 (IL-1), we have made several cDNA libraries from human umbilical vein endothelial cells (HUVEC) stimulated for 1 h with IL-1 in the presence of cycloheximide. The cDNA libraries were differentially screened with labelled cDNA derived from mRNA isolated from untreated or IL-1 treated HUVEC. Forty cDNA clones induced by IL-1 were isolated and partially sequenced. Thirty-eight of these corresponded to known genes, that is IL-8, ELAM-1, GRO-α, GRO-β, PA-I and I-kB. The last two clones contained an identical insert belonging to a previously unknown gene. The full length cDNA of this new gene was isolated and called PTX3. It encodes for a 42 kD, 381 amino acid long protein which shows in its 3' half a high degree of homology to all the known members of the pentaxin gene family including C-reactive protein (CRP) and serum amyloid P component (SAP). PTX3 may represent a novel marker of inflammatory reactions, particularly those involving the vessel wall.

Original languageEnglish
Pages (from-to)78-81
Number of pages4
JournalEuropean Heart Journal
Volume14
Issue numberSUPPL. K
Publication statusPublished - 1993

Keywords

  • Endothelial cells
  • Interleukin-1
  • Pentaxin

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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  • Cite this

    Introna, M., Breviario, F., D'Aniello, E. M., Golay, J., Dejana, E., & Mantovani, A. (1993). IL-1 inducible genes in human umbilical vein endothelial cells. European Heart Journal, 14(SUPPL. K), 78-81.