IL-10 and IL-4 co-operate to normalize in vitro IgA production in IgA- deficient (IgAD) patients

M. Marconi, A. Plebani, M. A. Avanzini, R. Maccario, A. Pistorio, M. Duse, M. Stringa, V. Monafo

Research output: Contribution to journalArticlepeer-review


In the present study we evaluated in vitro immunoglobulin production from IgAD individuals and healthy controls. Peripheral blood mononuclear cells (PBMC) from IgAD and controls were cultured with anti-CD40 MoAb presented on a CDw32-transfected fibroblast cell line (CD40 system) in the presence of IL-10, IL-2, IL-4, transforming growth factor-beta (TGF-β) alone as well as of IL-10 in combination with each of the other three cytokines. Only IL- 10 added alone induced significant changes in baseline immunoglobulin production; marked increases in median supernatant levels of all three isotypes were observed in both groups. The most striking finding of this study was the synergizing effect of IL-4 on IgA production in the IgAD group when added with IL-10; median IgA supernatant level increased to a value superimposable on that found in the normal controls which remained about the same as when stimulated with IL-10 alone. The synergic effect of IL-4 and IL-10 was specific to the IgA isotype.

Original languageEnglish
Pages (from-to)528-532
Number of pages5
JournalClinical and Experimental Immunology
Issue number3
Publication statusPublished - 1998


  • CD40 ligand
  • IgA deficiency
  • Immunoglobulin production
  • Interleukins

ASJC Scopus subject areas

  • Immunology


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