Low density mononuclear cells (LDMC) cultured with GM-CSF+IL-13 for 7 days differentiate into cells with the morphological features and functional activity of dendritic cells (DC). We have investigated the effect of IL-10 on the in vitro differentiation into DC. LDMC cultured with GMCSF+IL-13 and 20 ng/ml of IL-10 for 7 days did not expressed CDla, had a decreased ability to stimulate allogenic T cells in MLR and were morphologically different from control cultures. On the other hand IL-10 strongly stimulated pinocytosis of FITC-dextran, a function characteristic of immature DC and partly mediated by the mannose receptor (MR). In this view it is of interest that the expression of PAM-1, a MR specific mAb, was strongly upregulated in IL-10-cultured cells. EL-10 not only inhibited DC differentiation from LDMC but also favoured their differentiation into macrophages. In fact cells cultured for 7 days with GM-CSF+DL-13+IL10 developed into large cells, morphologically similar to macrophages and expressing high levels of CD14, CD16 and CD68. Istochemical studies revealed that these cells stained positively for non-specific esterase, similarly to monocyte-derived macrophages, while control DC cultures were ANAE negative. Thus, morphological, phenotypic, functional and istochemical studies support the conclusion that IL-10 inhibits DC differentiation from circulating precursors and shifts into the macrophage differentiation pathway.
|Publication status||Published - 1996|
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology