IL-10 polymorphisms are associated with early-onset celiac disease and severe mucosal damage in patients of Caucasian origin

Donatella Barisani, Stefano Ceroni, Raffaella Meneveri, Bruno M. Cesana, Maria Teresa Bardella

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Purpose: Polymorphisms in cytokine genes can determine their level of expression and affect the phenotypic expression of immunomediated diseases, such as celiac disease (CD). We thus evaluated cytokine polymorphism prevalence in CD patients and population controls, and assessed the correlation between specific polymorphisms and celiacs' clinical characteristics. Methods: 155 CD patients and 202 population controls were enrolled in the study. Cytokine polymorphisms (TNFα -308 and -238, IL-1β -511 and +3954, IL-1b RN + 2018, IL-6 -174, IL-10 -1082, -819 and -592, TGFβ1 + 29 and + 74, IFN-γ + 874) and HLA class II alleles were determined by sequence-specific primer polymerase chain reaction or restriction fragment length polymorphism analysis. Duodenal histology was classified using Marsh's criteria. Variables significantly associated with CD patients' characteristics were identified by multivariate logistic regression analysis. Results: A significantly higher frequency of -308 TNFα polymorphism was observed in CD patients compared to the entire population control group, although no difference was detected when population controls were stratified according to HLA class II. Among celiacs, early onset of the disease (≤2 year old) and the presence of a severe intestinal lesion (Marsh 3C) were significantly associated with the -1082 A/A IL-10 genotype which corresponds to a low producer phenotype (OR 3.28, 95% CI 1.49-7.19 and OR 2.60, 95% CI 1.04-6.49, respectively). Conclusion: The association between IL-10 genotypes and both histological severity at diagnosis and age of onset could be related to an alteration in cytokine balance, and supports the idea that the various clinical manifestations of the disease could be determined by a different genetic background.

Original languageEnglish
Pages (from-to)169-174
Number of pages6
JournalGenetics in Medicine
Volume8
Issue number3
DOIs
Publication statusPublished - Mar 2006

Fingerprint

Celiac Disease
Population Control
Interleukin-10
Cytokines
Wetlands
Abdomen
Genotype
Interleukin-1
Age of Onset
Population Groups
Restriction Fragment Length Polymorphisms
Interleukin-6
Histology
Logistic Models
Alleles
Regression Analysis
Phenotype
Polymerase Chain Reaction
Control Groups
Genes

Keywords

  • Celiac disease
  • Cytokine polymorphisms
  • IFN gamma
  • IL-10
  • TNF alpha

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

IL-10 polymorphisms are associated with early-onset celiac disease and severe mucosal damage in patients of Caucasian origin. / Barisani, Donatella; Ceroni, Stefano; Meneveri, Raffaella; Cesana, Bruno M.; Bardella, Maria Teresa.

In: Genetics in Medicine, Vol. 8, No. 3, 03.2006, p. 169-174.

Research output: Contribution to journalArticle

Barisani, Donatella ; Ceroni, Stefano ; Meneveri, Raffaella ; Cesana, Bruno M. ; Bardella, Maria Teresa. / IL-10 polymorphisms are associated with early-onset celiac disease and severe mucosal damage in patients of Caucasian origin. In: Genetics in Medicine. 2006 ; Vol. 8, No. 3. pp. 169-174.
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N2 - Purpose: Polymorphisms in cytokine genes can determine their level of expression and affect the phenotypic expression of immunomediated diseases, such as celiac disease (CD). We thus evaluated cytokine polymorphism prevalence in CD patients and population controls, and assessed the correlation between specific polymorphisms and celiacs' clinical characteristics. Methods: 155 CD patients and 202 population controls were enrolled in the study. Cytokine polymorphisms (TNFα -308 and -238, IL-1β -511 and +3954, IL-1b RN + 2018, IL-6 -174, IL-10 -1082, -819 and -592, TGFβ1 + 29 and + 74, IFN-γ + 874) and HLA class II alleles were determined by sequence-specific primer polymerase chain reaction or restriction fragment length polymorphism analysis. Duodenal histology was classified using Marsh's criteria. Variables significantly associated with CD patients' characteristics were identified by multivariate logistic regression analysis. Results: A significantly higher frequency of -308 TNFα polymorphism was observed in CD patients compared to the entire population control group, although no difference was detected when population controls were stratified according to HLA class II. Among celiacs, early onset of the disease (≤2 year old) and the presence of a severe intestinal lesion (Marsh 3C) were significantly associated with the -1082 A/A IL-10 genotype which corresponds to a low producer phenotype (OR 3.28, 95% CI 1.49-7.19 and OR 2.60, 95% CI 1.04-6.49, respectively). Conclusion: The association between IL-10 genotypes and both histological severity at diagnosis and age of onset could be related to an alteration in cytokine balance, and supports the idea that the various clinical manifestations of the disease could be determined by a different genetic background.

AB - Purpose: Polymorphisms in cytokine genes can determine their level of expression and affect the phenotypic expression of immunomediated diseases, such as celiac disease (CD). We thus evaluated cytokine polymorphism prevalence in CD patients and population controls, and assessed the correlation between specific polymorphisms and celiacs' clinical characteristics. Methods: 155 CD patients and 202 population controls were enrolled in the study. Cytokine polymorphisms (TNFα -308 and -238, IL-1β -511 and +3954, IL-1b RN + 2018, IL-6 -174, IL-10 -1082, -819 and -592, TGFβ1 + 29 and + 74, IFN-γ + 874) and HLA class II alleles were determined by sequence-specific primer polymerase chain reaction or restriction fragment length polymorphism analysis. Duodenal histology was classified using Marsh's criteria. Variables significantly associated with CD patients' characteristics were identified by multivariate logistic regression analysis. Results: A significantly higher frequency of -308 TNFα polymorphism was observed in CD patients compared to the entire population control group, although no difference was detected when population controls were stratified according to HLA class II. Among celiacs, early onset of the disease (≤2 year old) and the presence of a severe intestinal lesion (Marsh 3C) were significantly associated with the -1082 A/A IL-10 genotype which corresponds to a low producer phenotype (OR 3.28, 95% CI 1.49-7.19 and OR 2.60, 95% CI 1.04-6.49, respectively). Conclusion: The association between IL-10 genotypes and both histological severity at diagnosis and age of onset could be related to an alteration in cytokine balance, and supports the idea that the various clinical manifestations of the disease could be determined by a different genetic background.

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