IL-15 as memory T-cell adjuvant for topical HIV-1 DermaVir vaccine

Sandra A. Calarota, Anlan Dai, Jeffrey N. Trocio, David B. Weiner, Franco Lori, Julianna Lisziewicz

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

IL-7 and IL-15 are key cytokines involved in the generation and maintenance of memory CD8+ T-cells. We evaluated these cytokines as molecular adjuvants for topical HIV-1 DermaVir vaccine. We found that mice receiving DermaVir formulated with HIV-1 Gag plasmid in the presence of IL-7- or IL-15-encoding plasmid significantly enhanced Gag-specific central memory T-cells, as measured by a peptide-based cultured IFN-γ ELISPOT. Additionally, IL-15 significantly improved DermaVir-induced Gag-specific effector memory CD8+ T-cell responses, measured by standard IFN-γ ELISPOT. In a DermaVir prime/vaccinia vector boost regimen, the inclusion of IL-15 together with DermaVir significantly improved Gag-specific effector memory T-cell responses. Our study demonstrates that IL-15 is more potent than IL-7 in enhancing HIV-1-specific central memory T-cells induced by topical DermaVir. IL-15 adjuvanted DermaVir might be an alternative prime in a prophylactic vaccine regimen.

Original languageEnglish
Pages (from-to)5188-5195
Number of pages8
JournalVaccine
Volume26
Issue number40
DOIs
Publication statusPublished - Sep 19 2008

Fingerprint

Interleukin-15
Human immunodeficiency virus 1
adjuvants
HIV-1
interleukin-7
Vaccines
T-lymphocytes
vaccines
T-Lymphocytes
Interleukin-7
Enzyme-Linked Immunospot Assay
plasmids
cytokines
Vaccinium
Plasmids
Cytokines
Vaccinia
DermaVir
peptides
mice

Keywords

  • Cytokine adjuvant
  • Memory T-cells
  • Topical HIV-1 DermaVir

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Calarota, S. A., Dai, A., Trocio, J. N., Weiner, D. B., Lori, F., & Lisziewicz, J. (2008). IL-15 as memory T-cell adjuvant for topical HIV-1 DermaVir vaccine. Vaccine, 26(40), 5188-5195. https://doi.org/10.1016/j.vaccine.2008.03.067

IL-15 as memory T-cell adjuvant for topical HIV-1 DermaVir vaccine. / Calarota, Sandra A.; Dai, Anlan; Trocio, Jeffrey N.; Weiner, David B.; Lori, Franco; Lisziewicz, Julianna.

In: Vaccine, Vol. 26, No. 40, 19.09.2008, p. 5188-5195.

Research output: Contribution to journalArticle

Calarota, SA, Dai, A, Trocio, JN, Weiner, DB, Lori, F & Lisziewicz, J 2008, 'IL-15 as memory T-cell adjuvant for topical HIV-1 DermaVir vaccine', Vaccine, vol. 26, no. 40, pp. 5188-5195. https://doi.org/10.1016/j.vaccine.2008.03.067
Calarota SA, Dai A, Trocio JN, Weiner DB, Lori F, Lisziewicz J. IL-15 as memory T-cell adjuvant for topical HIV-1 DermaVir vaccine. Vaccine. 2008 Sep 19;26(40):5188-5195. https://doi.org/10.1016/j.vaccine.2008.03.067
Calarota, Sandra A. ; Dai, Anlan ; Trocio, Jeffrey N. ; Weiner, David B. ; Lori, Franco ; Lisziewicz, Julianna. / IL-15 as memory T-cell adjuvant for topical HIV-1 DermaVir vaccine. In: Vaccine. 2008 ; Vol. 26, No. 40. pp. 5188-5195.
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