IL-15 as memory T-cell adjuvant for topical HIV-1 DermaVir vaccine

Sandra A. Calarota, Anlan Dai, Jeffrey N. Trocio, David B. Weiner, Franco Lori, Julianna Lisziewicz

Research output: Contribution to journalArticlepeer-review

Abstract

IL-7 and IL-15 are key cytokines involved in the generation and maintenance of memory CD8+ T-cells. We evaluated these cytokines as molecular adjuvants for topical HIV-1 DermaVir vaccine. We found that mice receiving DermaVir formulated with HIV-1 Gag plasmid in the presence of IL-7- or IL-15-encoding plasmid significantly enhanced Gag-specific central memory T-cells, as measured by a peptide-based cultured IFN-γ ELISPOT. Additionally, IL-15 significantly improved DermaVir-induced Gag-specific effector memory CD8+ T-cell responses, measured by standard IFN-γ ELISPOT. In a DermaVir prime/vaccinia vector boost regimen, the inclusion of IL-15 together with DermaVir significantly improved Gag-specific effector memory T-cell responses. Our study demonstrates that IL-15 is more potent than IL-7 in enhancing HIV-1-specific central memory T-cells induced by topical DermaVir. IL-15 adjuvanted DermaVir might be an alternative prime in a prophylactic vaccine regimen.

Original languageEnglish
Pages (from-to)5188-5195
Number of pages8
JournalVaccine
Volume26
Issue number40
DOIs
Publication statusPublished - Sep 19 2008

Keywords

  • Cytokine adjuvant
  • Memory T-cells
  • Topical HIV-1 DermaVir

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

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