IL-15 cis Presentation Is Required for Optimal NK Cell Activation in Lipopolysaccharide-Mediated Inflammatory Conditions

Ivan Zanoni; Roberto Spreafico; Caterina Bodio; Marco DiGioia; Clara Cigni; Achille Broggi; Tatiana Gorletta; Michele Caccia; Giuseppe Chirico; Laura Sironi; Maddalena Collini; Mario P. Colombo; Natalio Garbi; Francesca Granucci

doi:10.1016/j.celrep.2013.08.021

IL-15 cis Presentation Is Required for Optimal NK Cell Activation in Lipopolysaccharide-Mediated Inflammatory Conditions

Ivan Zanoni, Roberto Spreafico, Caterina Bodio, Marco DiGioia, Clara Cigni, Achille Broggi, Tatiana Gorletta, Michele Caccia, Giuseppe Chirico, Laura Sironi, Maddalena Collini, Mario P. Colombo, Natalio Garbi, Francesca Granucci

Research output: Contribution to journal › Article › peer-review

Abstract

Natural killer (NK) cells have antitumor, antiviral, and antibacterial functions, and efforts are being made to manipulate them in immunotherapeutic approaches. However, their activation mechanisms remain poorly defined, particularly during bacterial infections. Here, we show that upon lipopolysaccharide or E.coli exposure, dendritic cells (DCs) produce three cytokines-interleukin 2 (IL-2), IL-18, and interferon β (IFN-β)-necessary and sufficient for NK cell activation. IFN-β enhances NK cell activation by inducing IL-15 and IL-15 receptor α not only in DCs but, surprisingly, also in NK cells. This process allows the transfer of IL-15 on NK cell surface and its cis presentation. cis-presented NK cell-derived and trans-presentedDC-derived IL-15 contribute equally to optimal NK cell activation

Original language	English
Pages (from-to)	1235-1249
Number of pages	15
Journal	Cell Reports
Volume	4
Issue number	6
DOIs	https://doi.org/10.1016/j.celrep.2013.08.021
Publication status	Published - Sep 26 2013

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/j.celrep.2013.08.021

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Zanoni, I., Spreafico, R., Bodio, C., DiGioia, M., Cigni, C., Broggi, A., Gorletta, T., Caccia, M., Chirico, G., Sironi, L., Collini, M., Colombo, M. P., Garbi, N., & Granucci, F. (2013). IL-15 cis Presentation Is Required for Optimal NK Cell Activation in Lipopolysaccharide-Mediated Inflammatory Conditions. Cell Reports, 4(6), 1235-1249. https://doi.org/10.1016/j.celrep.2013.08.021

IL-15 cis Presentation Is Required for Optimal NK Cell Activation in Lipopolysaccharide-Mediated Inflammatory Conditions. / Zanoni, Ivan; Spreafico, Roberto; Bodio, Caterina; DiGioia, Marco; Cigni, Clara; Broggi, Achille; Gorletta, Tatiana; Caccia, Michele; Chirico, Giuseppe; Sironi, Laura; Collini, Maddalena; Colombo, Mario P.; Garbi, Natalio; Granucci, Francesca.

In: Cell Reports, Vol. 4, No. 6, 26.09.2013, p. 1235-1249.

Research output: Contribution to journal › Article › peer-review

Zanoni, I, Spreafico, R, Bodio, C, DiGioia, M, Cigni, C, Broggi, A, Gorletta, T, Caccia, M, Chirico, G, Sironi, L, Collini, M, Colombo, MP, Garbi, N & Granucci, F 2013, 'IL-15 cis Presentation Is Required for Optimal NK Cell Activation in Lipopolysaccharide-Mediated Inflammatory Conditions', Cell Reports, vol. 4, no. 6, pp. 1235-1249. https://doi.org/10.1016/j.celrep.2013.08.021

Zanoni, Ivan ; Spreafico, Roberto ; Bodio, Caterina ; DiGioia, Marco ; Cigni, Clara ; Broggi, Achille ; Gorletta, Tatiana ; Caccia, Michele ; Chirico, Giuseppe ; Sironi, Laura ; Collini, Maddalena ; Colombo, Mario P. ; Garbi, Natalio ; Granucci, Francesca. / IL-15 cis Presentation Is Required for Optimal NK Cell Activation in Lipopolysaccharide-Mediated Inflammatory Conditions. In: Cell Reports. 2013 ; Vol. 4, No. 6. pp. 1235-1249.

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abstract = "Natural killer (NK) cells have antitumor, antiviral, and antibacterial functions, and efforts are being made to manipulate them in immunotherapeutic approaches. However, their activation mechanisms remain poorly defined, particularly during bacterial infections. Here, we show that upon lipopolysaccharide or E.coli exposure, dendritic cells (DCs) produce three cytokines-interleukin 2 (IL-2), IL-18, and interferon β (IFN-β)-necessary and sufficient for NK cell activation. IFN-β enhances NK cell activation by inducing IL-15 and IL-15 receptor α not only in DCs but, surprisingly, also in NK cells. This process allows the transfer of IL-15 on NK cell surface and its cis presentation. cis-presented NK cell-derived and trans-presentedDC-derived IL-15 contribute equally to optimal NK cell activation",

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AU - Cigni, Clara

AU - Broggi, Achille

AU - Gorletta, Tatiana

AU - Caccia, Michele

AU - Chirico, Giuseppe

AU - Sironi, Laura

AU - Collini, Maddalena

AU - Colombo, Mario P.

AU - Garbi, Natalio

AU - Granucci, Francesca

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AB - Natural killer (NK) cells have antitumor, antiviral, and antibacterial functions, and efforts are being made to manipulate them in immunotherapeutic approaches. However, their activation mechanisms remain poorly defined, particularly during bacterial infections. Here, we show that upon lipopolysaccharide or E.coli exposure, dendritic cells (DCs) produce three cytokines-interleukin 2 (IL-2), IL-18, and interferon β (IFN-β)-necessary and sufficient for NK cell activation. IFN-β enhances NK cell activation by inducing IL-15 and IL-15 receptor α not only in DCs but, surprisingly, also in NK cells. This process allows the transfer of IL-15 on NK cell surface and its cis presentation. cis-presented NK cell-derived and trans-presentedDC-derived IL-15 contribute equally to optimal NK cell activation

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IL-15 cis Presentation Is Required for Optimal NK Cell Activation in Lipopolysaccharide-Mediated Inflammatory Conditions

Abstract

ASJC Scopus subject areas

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