TY - JOUR
T1 - IL-15 cis Presentation Is Required for Optimal NK Cell Activation in Lipopolysaccharide-Mediated Inflammatory Conditions
AU - Zanoni, Ivan
AU - Spreafico, Roberto
AU - Bodio, Caterina
AU - DiGioia, Marco
AU - Cigni, Clara
AU - Broggi, Achille
AU - Gorletta, Tatiana
AU - Caccia, Michele
AU - Chirico, Giuseppe
AU - Sironi, Laura
AU - Collini, Maddalena
AU - Colombo, Mario P.
AU - Garbi, Natalio
AU - Granucci, Francesca
PY - 2013/9/26
Y1 - 2013/9/26
N2 - Natural killer (NK) cells have antitumor, antiviral, and antibacterial functions, and efforts are being made to manipulate them in immunotherapeutic approaches. However, their activation mechanisms remain poorly defined, particularly during bacterial infections. Here, we show that upon lipopolysaccharide or E.coli exposure, dendritic cells (DCs) produce three cytokines-interleukin 2 (IL-2), IL-18, and interferon β (IFN-β)-necessary and sufficient for NK cell activation. IFN-β enhances NK cell activation by inducing IL-15 and IL-15 receptor α not only in DCs but, surprisingly, also in NK cells. This process allows the transfer of IL-15 on NK cell surface and its cis presentation. cis-presented NK cell-derived and trans-presentedDC-derived IL-15 contribute equally to optimal NK cell activation
AB - Natural killer (NK) cells have antitumor, antiviral, and antibacterial functions, and efforts are being made to manipulate them in immunotherapeutic approaches. However, their activation mechanisms remain poorly defined, particularly during bacterial infections. Here, we show that upon lipopolysaccharide or E.coli exposure, dendritic cells (DCs) produce three cytokines-interleukin 2 (IL-2), IL-18, and interferon β (IFN-β)-necessary and sufficient for NK cell activation. IFN-β enhances NK cell activation by inducing IL-15 and IL-15 receptor α not only in DCs but, surprisingly, also in NK cells. This process allows the transfer of IL-15 on NK cell surface and its cis presentation. cis-presented NK cell-derived and trans-presentedDC-derived IL-15 contribute equally to optimal NK cell activation
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UR - http://www.scopus.com/inward/citedby.url?scp=84884558141&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2013.08.021
DO - 10.1016/j.celrep.2013.08.021
M3 - Article
C2 - 24055061
AN - SCOPUS:84884558141
VL - 4
SP - 1235
EP - 1249
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 6
ER -