IL-15/IL-15Rα intracellular trafficking in human cells and protection from apoptosis

Raffaele Pereno, Alessia Gaggero, Marco Scudeletti, Lorella Lanza, Raffaella Meazza, Zohar Mishal, Claude Jasmin, Francesco Indiveri, Silvano Ferrini, Bruno Azzarone

Research output: Contribution to journalArticle

Abstract

IL-15 is an immunostimulatory cytokine sharing with IL-2 the IL-2Rβγ complex. In vivo, IL-15 detection in synovial fluids has been associated with the development of rheumatoid arthritis. A debate exists as to whether IL-15 has the potential to be secreted in meaningful amounts or to act as a pericellular cytokine. Our data show (1) the presence of two IL-15 isoforms displaying signal peptides of different length and the capacity to be secreted restricted to the isoform bearing the longer one; (2) in cells expressing the two isoforms, the existence of different nuclear localization and intracellular trafficking of IL-15 and IL-15Rα; and (3) an intercellular microcirculation of IL-15, not detectable with ELISA kits, but displaying a role as an anti-apoptotic factor able to induce the deflection of the TNFR associated factor 2 (TRAF) to IL-15Rα. Our data point to a juxtacrine mechanism of action of IL-15 and suggest a role for IL-15/IL-15Rα in the regulation of apoptosis.

Original languageEnglish
Pages (from-to)236-245
Number of pages10
JournalAnnals of the New York Academy of Sciences
Volume876
DOIs
Publication statusPublished - 1999

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Human Trafficking
Interleukin-15
Cytoprotection
Cells
Apoptosis
Protein Isoforms
Interleukin-2
Bearings (structural)
Cytokines
Microcirculation
Localization
Deflection
Length
Datum Point
Interleukin-3
Synovial Fluid
Protein Sorting Signals
Rheumatoid Arthritis
Enzyme-Linked Immunosorbent Assay

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

IL-15/IL-15Rα intracellular trafficking in human cells and protection from apoptosis. / Pereno, Raffaele; Gaggero, Alessia; Scudeletti, Marco; Lanza, Lorella; Meazza, Raffaella; Mishal, Zohar; Jasmin, Claude; Indiveri, Francesco; Ferrini, Silvano; Azzarone, Bruno.

In: Annals of the New York Academy of Sciences, Vol. 876, 1999, p. 236-245.

Research output: Contribution to journalArticle

Pereno, R, Gaggero, A, Scudeletti, M, Lanza, L, Meazza, R, Mishal, Z, Jasmin, C, Indiveri, F, Ferrini, S & Azzarone, B 1999, 'IL-15/IL-15Rα intracellular trafficking in human cells and protection from apoptosis', Annals of the New York Academy of Sciences, vol. 876, pp. 236-245. https://doi.org/10.1111/j.1749-6632.1999.tb07644.x
Pereno, Raffaele ; Gaggero, Alessia ; Scudeletti, Marco ; Lanza, Lorella ; Meazza, Raffaella ; Mishal, Zohar ; Jasmin, Claude ; Indiveri, Francesco ; Ferrini, Silvano ; Azzarone, Bruno. / IL-15/IL-15Rα intracellular trafficking in human cells and protection from apoptosis. In: Annals of the New York Academy of Sciences. 1999 ; Vol. 876. pp. 236-245.
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