IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes

Davide Pennino, Kilian Eyerich, Claudia Scarponi, Teresa Carbone, Stefanie Eyerich, Francesca Nasorri, Simone Garcovich, Claudia Traidl-Hoffmann, Cristina Albanesi, Andrea Cavani

Research output: Contribution to journalArticle

Abstract

Th17 is a newly identified lineage of effector T cells involved in autoimmunity and immune responses to pathogens. We demonstrate in this study the pathogenic role of IL-17-producing CD4 + T lymphocytes in allergic contact dermatitis (ACD) to skinapplied chemicals. IL-17 + T cells infiltrate ACD reactions and predominantly distribute at the site of heavy spongiosis. Skin IL-17 + T cells were functionally and phenotypically heterogeneous: although pure Th17 prevailed in ACD skin, hapten responsiveness was restricted to Th1/IL-17 (IFN-γ +IL-17 +) and Th0/IL-17 (IFN-γ +IL-17 +IL-4 +) fractions, and to lesser extent Th2/ IL-17 cells. In the IFN-γ-dominated ACD environment, IL-17-releasing T cells affect immune function of keratinocytes by promoting CXCL8, IL-6, and HBD-2 production. In addition, compared with Th1, supernatants from Th1/IL-17 T cells were much more efficient in inducing ICAM-1 expression on keratinocytes and keratinocyte-T cell adhesiveness in vitro. As a consequence, exposure to combined IFN-g and IL-17 rendered keratinocytes susceptible to ICAM-1-dependent Ag non-specific T cell killing. Thus, IL-17 efficiently amplifies the allergic reaction by rendering virtually all of the T lymphocytes recruited at the site of skin inflammation capable to directly contribute to tissue damage.

Original languageEnglish
Pages (from-to)4880-4888
Number of pages9
JournalJournal of Immunology
Volume184
Issue number9
DOIs
Publication statusPublished - May 1 2010

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

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  • Cite this

    Pennino, D., Eyerich, K., Scarponi, C., Carbone, T., Eyerich, S., Nasorri, F., Garcovich, S., Traidl-Hoffmann, C., Albanesi, C., & Cavani, A. (2010). IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes. Journal of Immunology, 184(9), 4880-4888. https://doi.org/10.4049/jimmunol.0901767