IL-18 level in patients undergoing coronary artery bypass grafting surgery or valve replacement: Which link with epicardial fat depot?

E. Dozio, G. Dogliotti, A. E. Malavazos, F. Bandera, G. Cassetti, E. Vianello, R. Zelaschi, A. Barassi, G. Pellissero, U. Solimene, L. Morricone, A. Sigruener, V. Tarabin, G. Schmitz, L. Menicanti, M. M. Corsi Romanelli

Research output: Contribution to journalArticle

Abstract

Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Due to the link between obesity, inflammation and cardiovascular diseases, we aimed to measure IL-18 circulating level in patients undergoing open-heart surgery both for elective coronary artery bypass grafting (CABG) or for valve replacement (VR), and we also evaluated whether epicardial adipose tissue (EAT) depot may be a potential source of IL-18. Circulating IL-18 protein was quantified by enzyme-linked immunosorbent assay. IL-18, IL-18 receptor 1 (IL-18 R1) and IL-18 receptor accessory protein (IL-18-RAP) gene expression in EAT depot were evaluated by one colour microarray platform. EAT thickness was measured by echocardiography. In this study we found that all cardiovascular patients (CABG and VR) have increased circulating IL-18 level compared to healthy control subjects (p <0.0001), but no statistical significant difference was observed between CABG and VR groups (p = 0.35). A great increase in the gene expression of IL-18 (p <0.05), IL-18 R1 (p <0.01) and IL-18 RAP (p <0.001) was observed in EAT samples obtained from CABG vs VR patients. In conclusion, CABG and VR patients had similar increased level of circulating IL-18 protein, but in EAT depots isolated from CABG gene expression of IL-18, IL-18 R1 and IL-18-RAP resulted higher than in VR patients. Future investigation on local IL-18 protein production, its autocrine-paracrine effect and its correlation with plasmatic IL-18 level could give more information on the relationship between IL-18 and coronary artery disease.

Original languageEnglish
Pages (from-to)1011-1020
Number of pages10
JournalInternational Journal of Immunopathology and Pharmacology
Volume25
Issue number4
Publication statusPublished - Oct 2012

Fingerprint

Interleukin-18
Coronary Artery Bypass
Fats
Adipose Tissue
Interleukin-18 Receptor beta Subunit
Gene Expression
Interleukin-18 Receptor alpha Subunit
Proteins
Interleukin-1

Keywords

  • Adiposity
  • Cardiovascular diseases
  • Coronary artery bypass grafting surgery
  • Epicardial fat depot
  • Interleukin-18
  • Valve replacement

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy

Cite this

IL-18 level in patients undergoing coronary artery bypass grafting surgery or valve replacement : Which link with epicardial fat depot? / Dozio, E.; Dogliotti, G.; Malavazos, A. E.; Bandera, F.; Cassetti, G.; Vianello, E.; Zelaschi, R.; Barassi, A.; Pellissero, G.; Solimene, U.; Morricone, L.; Sigruener, A.; Tarabin, V.; Schmitz, G.; Menicanti, L.; Corsi Romanelli, M. M.

In: International Journal of Immunopathology and Pharmacology, Vol. 25, No. 4, 10.2012, p. 1011-1020.

Research output: Contribution to journalArticle

Dozio, E, Dogliotti, G, Malavazos, AE, Bandera, F, Cassetti, G, Vianello, E, Zelaschi, R, Barassi, A, Pellissero, G, Solimene, U, Morricone, L, Sigruener, A, Tarabin, V, Schmitz, G, Menicanti, L & Corsi Romanelli, MM 2012, 'IL-18 level in patients undergoing coronary artery bypass grafting surgery or valve replacement: Which link with epicardial fat depot?', International Journal of Immunopathology and Pharmacology, vol. 25, no. 4, pp. 1011-1020.
Dozio, E. ; Dogliotti, G. ; Malavazos, A. E. ; Bandera, F. ; Cassetti, G. ; Vianello, E. ; Zelaschi, R. ; Barassi, A. ; Pellissero, G. ; Solimene, U. ; Morricone, L. ; Sigruener, A. ; Tarabin, V. ; Schmitz, G. ; Menicanti, L. ; Corsi Romanelli, M. M. / IL-18 level in patients undergoing coronary artery bypass grafting surgery or valve replacement : Which link with epicardial fat depot?. In: International Journal of Immunopathology and Pharmacology. 2012 ; Vol. 25, No. 4. pp. 1011-1020.
@article{8720bf70406848908e7bfd8a2d4acc6e,
title = "IL-18 level in patients undergoing coronary artery bypass grafting surgery or valve replacement: Which link with epicardial fat depot?",
abstract = "Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Due to the link between obesity, inflammation and cardiovascular diseases, we aimed to measure IL-18 circulating level in patients undergoing open-heart surgery both for elective coronary artery bypass grafting (CABG) or for valve replacement (VR), and we also evaluated whether epicardial adipose tissue (EAT) depot may be a potential source of IL-18. Circulating IL-18 protein was quantified by enzyme-linked immunosorbent assay. IL-18, IL-18 receptor 1 (IL-18 R1) and IL-18 receptor accessory protein (IL-18-RAP) gene expression in EAT depot were evaluated by one colour microarray platform. EAT thickness was measured by echocardiography. In this study we found that all cardiovascular patients (CABG and VR) have increased circulating IL-18 level compared to healthy control subjects (p <0.0001), but no statistical significant difference was observed between CABG and VR groups (p = 0.35). A great increase in the gene expression of IL-18 (p <0.05), IL-18 R1 (p <0.01) and IL-18 RAP (p <0.001) was observed in EAT samples obtained from CABG vs VR patients. In conclusion, CABG and VR patients had similar increased level of circulating IL-18 protein, but in EAT depots isolated from CABG gene expression of IL-18, IL-18 R1 and IL-18-RAP resulted higher than in VR patients. Future investigation on local IL-18 protein production, its autocrine-paracrine effect and its correlation with plasmatic IL-18 level could give more information on the relationship between IL-18 and coronary artery disease.",
keywords = "Adiposity, Cardiovascular diseases, Coronary artery bypass grafting surgery, Epicardial fat depot, Interleukin-18, Valve replacement",
author = "E. Dozio and G. Dogliotti and Malavazos, {A. E.} and F. Bandera and G. Cassetti and E. Vianello and R. Zelaschi and A. Barassi and G. Pellissero and U. Solimene and L. Morricone and A. Sigruener and V. Tarabin and G. Schmitz and L. Menicanti and {Corsi Romanelli}, {M. M.}",
year = "2012",
month = "10",
language = "English",
volume = "25",
pages = "1011--1020",
journal = "International Journal of Immunopathology and Pharmacology",
issn = "0394-6320",
publisher = "Biomedical Research Press s.a.s.",
number = "4",

}

TY - JOUR

T1 - IL-18 level in patients undergoing coronary artery bypass grafting surgery or valve replacement

T2 - Which link with epicardial fat depot?

AU - Dozio, E.

AU - Dogliotti, G.

AU - Malavazos, A. E.

AU - Bandera, F.

AU - Cassetti, G.

AU - Vianello, E.

AU - Zelaschi, R.

AU - Barassi, A.

AU - Pellissero, G.

AU - Solimene, U.

AU - Morricone, L.

AU - Sigruener, A.

AU - Tarabin, V.

AU - Schmitz, G.

AU - Menicanti, L.

AU - Corsi Romanelli, M. M.

PY - 2012/10

Y1 - 2012/10

N2 - Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Due to the link between obesity, inflammation and cardiovascular diseases, we aimed to measure IL-18 circulating level in patients undergoing open-heart surgery both for elective coronary artery bypass grafting (CABG) or for valve replacement (VR), and we also evaluated whether epicardial adipose tissue (EAT) depot may be a potential source of IL-18. Circulating IL-18 protein was quantified by enzyme-linked immunosorbent assay. IL-18, IL-18 receptor 1 (IL-18 R1) and IL-18 receptor accessory protein (IL-18-RAP) gene expression in EAT depot were evaluated by one colour microarray platform. EAT thickness was measured by echocardiography. In this study we found that all cardiovascular patients (CABG and VR) have increased circulating IL-18 level compared to healthy control subjects (p <0.0001), but no statistical significant difference was observed between CABG and VR groups (p = 0.35). A great increase in the gene expression of IL-18 (p <0.05), IL-18 R1 (p <0.01) and IL-18 RAP (p <0.001) was observed in EAT samples obtained from CABG vs VR patients. In conclusion, CABG and VR patients had similar increased level of circulating IL-18 protein, but in EAT depots isolated from CABG gene expression of IL-18, IL-18 R1 and IL-18-RAP resulted higher than in VR patients. Future investigation on local IL-18 protein production, its autocrine-paracrine effect and its correlation with plasmatic IL-18 level could give more information on the relationship between IL-18 and coronary artery disease.

AB - Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Due to the link between obesity, inflammation and cardiovascular diseases, we aimed to measure IL-18 circulating level in patients undergoing open-heart surgery both for elective coronary artery bypass grafting (CABG) or for valve replacement (VR), and we also evaluated whether epicardial adipose tissue (EAT) depot may be a potential source of IL-18. Circulating IL-18 protein was quantified by enzyme-linked immunosorbent assay. IL-18, IL-18 receptor 1 (IL-18 R1) and IL-18 receptor accessory protein (IL-18-RAP) gene expression in EAT depot were evaluated by one colour microarray platform. EAT thickness was measured by echocardiography. In this study we found that all cardiovascular patients (CABG and VR) have increased circulating IL-18 level compared to healthy control subjects (p <0.0001), but no statistical significant difference was observed between CABG and VR groups (p = 0.35). A great increase in the gene expression of IL-18 (p <0.05), IL-18 R1 (p <0.01) and IL-18 RAP (p <0.001) was observed in EAT samples obtained from CABG vs VR patients. In conclusion, CABG and VR patients had similar increased level of circulating IL-18 protein, but in EAT depots isolated from CABG gene expression of IL-18, IL-18 R1 and IL-18-RAP resulted higher than in VR patients. Future investigation on local IL-18 protein production, its autocrine-paracrine effect and its correlation with plasmatic IL-18 level could give more information on the relationship between IL-18 and coronary artery disease.

KW - Adiposity

KW - Cardiovascular diseases

KW - Coronary artery bypass grafting surgery

KW - Epicardial fat depot

KW - Interleukin-18

KW - Valve replacement

UR - http://www.scopus.com/inward/record.url?scp=84872902799&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872902799&partnerID=8YFLogxK

M3 - Article

C2 - 23298491

AN - SCOPUS:84872902799

VL - 25

SP - 1011

EP - 1020

JO - International Journal of Immunopathology and Pharmacology

JF - International Journal of Immunopathology and Pharmacology

SN - 0394-6320

IS - 4

ER -