IL-2 enhances c-fms expression in human monocytes

I. Espinoza-Delgado, D. L. Longo, G. L. Gusella, L. Varesio

Research output: Contribution to journalArticle

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Abstract

We investigated the effects of IL-2 and IFN-γ on the expression of the proto-oncogene c-fms mRNA, which encodes for the macrophage CSF receptor. Low constitutive expression of c-fms mRNA was detected in fresh human monocytes. Stimulation of monocytes with IL-2 induced a significant increase in c-fms mRNA relative to medium control that was observed as early as 6 h after IL-2 stimulation. Dose-response experiments showed that 100 U/ml of IL-2 were sufficient to enhance the expression of c-fms mRNA. In contrast IFN-γ did not modify the levels of c-fms transcript. Immunoprecipitation experiments demonstrated that IL-2 enhanced c-fms glycoprotein levels. Experiments in which monocytes were activated with IFN-γ or IL-2 followed by macrophage CSF-1 and then tested for tumoricidal activity demonstrated that macrophage CSF-1 sustained the cytotoxic activity induced by IL-2 but not by IFN-γ. These data demonstrate that IL-2 enhances c-fms mRNA and c-fms glycoprotein expression suggesting that IL-2, by augmenting expression of macrophage CSF-1 receptors, can lead to prolongation of monocyte-mediated tumoricidal activity obtained in the presence of exogenous macrophage CSF-1.

Original languageEnglish
Pages (from-to)1137-1143
Number of pages7
JournalJournal of Immunology
Volume145
Issue number4
Publication statusPublished - 1990

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Interleukin-2
Monocytes
Macrophages
Macrophage Colony-Stimulating Factor
Messenger RNA
Glycoproteins
fms Genes
Colony-Stimulating Factor Receptors
Macrophage Colony-Stimulating Factor Receptors
Immunoprecipitation

ASJC Scopus subject areas

  • Immunology

Cite this

Espinoza-Delgado, I., Longo, D. L., Gusella, G. L., & Varesio, L. (1990). IL-2 enhances c-fms expression in human monocytes. Journal of Immunology, 145(4), 1137-1143.

IL-2 enhances c-fms expression in human monocytes. / Espinoza-Delgado, I.; Longo, D. L.; Gusella, G. L.; Varesio, L.

In: Journal of Immunology, Vol. 145, No. 4, 1990, p. 1137-1143.

Research output: Contribution to journalArticle

Espinoza-Delgado, I, Longo, DL, Gusella, GL & Varesio, L 1990, 'IL-2 enhances c-fms expression in human monocytes', Journal of Immunology, vol. 145, no. 4, pp. 1137-1143.
Espinoza-Delgado I, Longo DL, Gusella GL, Varesio L. IL-2 enhances c-fms expression in human monocytes. Journal of Immunology. 1990;145(4):1137-1143.
Espinoza-Delgado, I. ; Longo, D. L. ; Gusella, G. L. ; Varesio, L. / IL-2 enhances c-fms expression in human monocytes. In: Journal of Immunology. 1990 ; Vol. 145, No. 4. pp. 1137-1143.
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