IL-2 induction of IL-1β mRNA expression in monocytes. Regulation by agents that block second messenger pathways

E. J. Kovacs, B. Brock, L. Varesio, H. A. Young

Research output: Contribution to journalArticle

Abstract

We have previously shown that in mixed cultures of PBL incubation with human rIL-2 induces the rapid expression of IL-1α and IL-1β mRNA. Because studies have demonstrated that IL-2R can be expressed on the surface of human peripheral blood monocytes, we chose to investigate whether IL-1β mRNA could be directly induced in purified human monocytes by treatment with IL-2 and, if so, to analyze the second messenger pathways by which it may be controlled. Human monocytes do not spontaneously express IL-1β mRNA, but can express the gene as soon as 1 h after treatment with IL-2. The level of IL-1β mRNA induced by IL-2 at 5 h in human monocytes was about one-fourth that induced by LPS. LPS induction of IL-1β mRNA in human monocytes can be blocked by either an inhibitor of protein kinase C (PKc) 1-(5-isoquinolinesulfonyl)-2-methylpiperazine or an inhibitor of calcium/calmodulin (CaM) kinase N-(6-aminohexyl)-5-chloro-1-naphtanesulfonamide, suggesting that both PKc and CaM kinase are involved in transducing signals initiated by LPS. In contrast, IL-2 induction of IL-1β mRNA expression is blocked only by 1-(5-isoquinolinesulfonyl)-2-methylpiperazine, suggesting that PKc, and not CaM kinase, is activated by IL-2. These data suggest that overlapping but distinct second messenger pathways are involved in the transduction of signals initiated by IL-2 and LPS.

Original languageEnglish
Pages (from-to)3532-3537
Number of pages6
JournalJournal of Immunology
Volume143
Issue number11
Publication statusPublished - 1989

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Second Messenger Systems
Interleukin-1
Interleukin-2
Monocytes
Messenger RNA
Calcium-Calmodulin-Dependent Protein Kinases
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Protein Kinase C
Signal Transduction
Calcium
Genes

ASJC Scopus subject areas

  • Immunology

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IL-2 induction of IL-1β mRNA expression in monocytes. Regulation by agents that block second messenger pathways. / Kovacs, E. J.; Brock, B.; Varesio, L.; Young, H. A.

In: Journal of Immunology, Vol. 143, No. 11, 1989, p. 3532-3537.

Research output: Contribution to journalArticle

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