IL-2 production by myofibroblasts from post-radiation fibrosis in breast cancer patients

A. Alileche, D. Han, S. Plaisance, E. Assier, Y. Sahraoui, C. Clemanceau, D. Metivier, D. Brouty-Boyer, C. Jasmin, B. Azzarone

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Abstract

The origin of cell activation in post-radiation fibrosis and its chronic extension are still poorly understood. Since local IL-2 cancer treatment sometimes triggers intraperitoneal fibrosis we have analyzed three myofibroblastic cell strains from post-radiation skin fibrosis (FPR7, FPR10 and FPR15) for their interactions with IL-2. In these cells we have observed the surface expression of the two chains of the IL-2R (IL-2Rαβ), the presence of the 0.9 kb transcript specific for the IL-2 gene and, by flow cytometry with anti-IL-2 mAbs, the presence of IL-2 immunoreactive material inside the cells up to 8 days after subculture. The FPR cell lines secreted IL-2, as determined by ELISA. The secreted IL-2 is biologically active since it sustains the proliferation of the IL-2-dependent murine lymphoid cell line CTLL2 and preincubation with anti-IL-2 blocking mAbs completely abolishes this activity. Overnight incubation of FPR cells with polyclonal anti-IL-2 antibodies leads to a decreased expression of the membrane adhesion molecules ICAM-1 and CD44, suggesting the existence of an autocrine/paracrine loop involved in the surface expression of these antigens. By contrast, in normal adult skin fibroblasts we did not detect IL-2 gene activation. In vivo, IL-2 secretion by post-radiation fibrosis fibroblasts and the subsequent up-regulation of ICAM-1 and CD44 may represent key events during the process that leads to radiation fibrosis.

Original languageEnglish
Pages (from-to)1585-1591
Number of pages7
JournalInternational Immunology
Volume6
Issue number10
Publication statusPublished - 1994

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ASJC Scopus subject areas

  • Immunology
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Neuropsychology and Physiological Psychology
  • Transplantation

Cite this

Alileche, A., Han, D., Plaisance, S., Assier, E., Sahraoui, Y., Clemanceau, C., Metivier, D., Brouty-Boyer, D., Jasmin, C., & Azzarone, B. (1994). IL-2 production by myofibroblasts from post-radiation fibrosis in breast cancer patients. International Immunology, 6(10), 1585-1591.