IL-2-Regulated Expression of the Monocyte Chemotactic Protein-1 Receptor (CCR2) in Human NK Cells: Characterization of a Predominant 3.4-Kilobase Transcript Containing CCR2B and CCR2A Sequences

Nadia Polentarutti, Paola Allavena, Giancarlo Bianchi, Giuseppina Giardina, Andrea Basile, Silvano Sozzani, Alberto Mantovani, Martino Introna

Research output: Contribution to journalArticlepeer-review

Abstract

NK cells migrate in response to C-C chemokines, including monocyte chemotactic protein-1 (MCP-1) and MCP-3. Increased migration was observed in IL-2-activated NK cells. It was therefore of interest to define the expression in resting and activated NK cells of the MCP-1 receptor (CCR2) for which two cDNAs (A and B) have been described. Specific oligonucleotides and reverse-transcriptase PCR revealed the presence in activated NK cells and mononuclear phagocytes of the fragments expected on the basis of the reported cDNAs. In addition, amplification with a common A/B- and an A-specific oligonucleotide yielded an unexpected, abundant, 1649-bp fragment. Sequence analysis as well as Northern blotting and RNase protection with different probes revealed that the CCR2 gene is expressed in activated NK cells and mononuclear phagocytes as a predominant long transcript (3.4 kb) consisting of CCR2B, followed by a novel sequence (X), corresponding to an intron in the genome, and by a CCR2A-specific portion. The predominant long transcript is polyadenylated and present in the cytoplasm. The augmented migratory capacity of IL-2 activated vs resting NK cells was associated with increased CCR2 transcript levels.

Original languageEnglish
Pages (from-to)2689-2694
Number of pages6
JournalJournal of Immunology
Volume158
Issue number6
Publication statusPublished - Mar 15 1997

ASJC Scopus subject areas

  • Immunology

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