IL-2 signaling in human monocytes involves the phosphorylation and activation of p59(hck)

Maria C. Bosco, Rafael E. Curiel, Arnold H. Zea, Maria G. Malabarba, John R. Ortaldo, Igor Espinoza-Delgado

Research output: Contribution to journalArticle

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Abstract

The activating properties of IL-2 and the structure of the IL-2R on human monocytes are well characterized. However, relatively little is known about the biochemical mechanisms involved in IL-2 signal transduction in these cells. We investigated the role of protein tyrosine kinases (PTKs) in the activation of monocytes by IL-2. Incubation of monocytes with the PTK inhibitor herbimycin A (HA) resulted in the dose-dependent suppression of IL- 2-induced monocyte tumoricidal activity. This inhibition was rather potent, as a concentration of HA as low as 0.5 μM caused a complete abrogation of cytolytic activity. Furthermore, HA markedly suppressed the ability of IL-2 to induce IL-1β, TNF-α, IL-6, and IL-8 mRNA expression and protein secretion by monocytes. Anti-phosphotyrosine immunoblotting demonstrated that IL-2 induced a rapid and time-dependent increase in tyrosine phosphorylation of several cellular proteins of molecular masses ranging from 35 to 180 kDa. Interestingly, IL-2 caused a significant up-regulation of the constitutive levels of hck PTK mRNA and protein relative to medium-treated cells as well as an increase in p59(hck) tyrosine phosphorylation. Finally, we demonstrated by in vitro kinase assay that the specific activity of p59(hck) PTK was also induced by IL-2 in monocytes. Thus, these data show that the activation of PTKs is required for the triggering of monocyte effector and secretory functions by IL-2 and strongly suggest that p59(hck) is a key participant in IL-2 signaling in human monocytes.

Original languageEnglish
Pages (from-to)4575-4585
Number of pages11
JournalJournal of Immunology
Volume164
Issue number9
Publication statusPublished - May 1 2000

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Interleukin-2
Monocytes
Phosphorylation
Protein-Tyrosine Kinases
Tyrosine
Messenger RNA
Phosphotyrosine
Proteins
Protein Kinase Inhibitors
Interleukin-8
Interleukin-1
Immunoblotting
Interleukin-6
Signal Transduction
Phosphotransferases
Up-Regulation

ASJC Scopus subject areas

  • Immunology

Cite this

Bosco, M. C., Curiel, R. E., Zea, A. H., Malabarba, M. G., Ortaldo, J. R., & Espinoza-Delgado, I. (2000). IL-2 signaling in human monocytes involves the phosphorylation and activation of p59(hck). Journal of Immunology, 164(9), 4575-4585.

IL-2 signaling in human monocytes involves the phosphorylation and activation of p59(hck). / Bosco, Maria C.; Curiel, Rafael E.; Zea, Arnold H.; Malabarba, Maria G.; Ortaldo, John R.; Espinoza-Delgado, Igor.

In: Journal of Immunology, Vol. 164, No. 9, 01.05.2000, p. 4575-4585.

Research output: Contribution to journalArticle

Bosco, MC, Curiel, RE, Zea, AH, Malabarba, MG, Ortaldo, JR & Espinoza-Delgado, I 2000, 'IL-2 signaling in human monocytes involves the phosphorylation and activation of p59(hck)', Journal of Immunology, vol. 164, no. 9, pp. 4575-4585.
Bosco MC, Curiel RE, Zea AH, Malabarba MG, Ortaldo JR, Espinoza-Delgado I. IL-2 signaling in human monocytes involves the phosphorylation and activation of p59(hck). Journal of Immunology. 2000 May 1;164(9):4575-4585.
Bosco, Maria C. ; Curiel, Rafael E. ; Zea, Arnold H. ; Malabarba, Maria G. ; Ortaldo, John R. ; Espinoza-Delgado, Igor. / IL-2 signaling in human monocytes involves the phosphorylation and activation of p59(hck). In: Journal of Immunology. 2000 ; Vol. 164, No. 9. pp. 4575-4585.
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