IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis

Roberta Caruso, Daniele Fina, Ilaria Peluso, Massimo Claudio Fantini, Claudio Tosti, Giovanna Del Vecchio Blanco, Omero Alessandro Paoluzi, Flavio Caprioli, Fabio Andrei, Carmine Stolfi, Marco Romano, Vittorio Ricci, Thomas T. MacDonald, Francesco Pallone, Giovanni Monteleone

Research output: Contribution to journalArticle

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Abstract

Helicobacter pylori (Hp) infection is associated with gastric inflammation and ulceration. The pathways of tissue damage in Hp-infected subjects are complex, but evidence indicates that T cell-derived cytokines enhance the synthesis of matrix metalloproteinases (MMP) that contribute to mucosal ulceration and epithelial damage. In this study, we have examined the role of the T cell cytokine IL-21 in Hp-infected gastric mucosa and evaluated whether IL-21 regulates MMP production by gastric epithelial cells. We show that IL-21 is constitutively expressed in gastric mucosa and is more abundant in biopsy specimens and purified mucosal CD3+ T cells from Hp-infected patients compared with normal patients and disease controls. We also demonstrate that IL-21R is expressed by primary gastric epithelial cells, as well as by the gastric epithelial cell lines AGS and MKN28. Consistently, AGS cells respond to IL-21 by increasing production of MMP-2 and MMP-9, but not MMP-1, MMP-3, MMP-7, or tissue inhibitors of MMP. Analysis of signaling pathways leading to MMP production reveals that IL-21 enhances NF-κB but not MAPK activation, and inhibition of NF-κB activation reduces IL-21-induced MMP-2 and MMP-9 production. Finally, we show that treatment of Hp-infected gastric explants with anti-IL-21 reduces epithelial cell-derived MMP-2 and MMP-9 production. These data indicate that IL-21 is overexpressed in Hp-infected gastric mucosa where it could contribute to increased epithelial gelatinase production.

Original languageEnglish
Pages (from-to)5957-5965
Number of pages9
JournalJournal of Immunology
Volume178
Issue number9
Publication statusPublished - May 1 2007

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Gelatinases
Gastric Mucosa
Helicobacter pylori
Stomach
Matrix Metalloproteinases
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Epithelial Cells
T-Lymphocytes
Matrix Metalloproteinase 7
Cytokines
Tissue Inhibitor of Metalloproteinases
Matrix Metalloproteinase 3
Matrix Metalloproteinase 1
Matrix Metalloproteinase Inhibitors
interleukin-21
Helicobacter Infections
Inflammation
Biopsy
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Caruso, R., Fina, D., Peluso, I., Fantini, M. C., Tosti, C., Blanco, G. D. V., ... Monteleone, G. (2007). IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis. Journal of Immunology, 178(9), 5957-5965.

IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis. / Caruso, Roberta; Fina, Daniele; Peluso, Ilaria; Fantini, Massimo Claudio; Tosti, Claudio; Blanco, Giovanna Del Vecchio; Paoluzi, Omero Alessandro; Caprioli, Flavio; Andrei, Fabio; Stolfi, Carmine; Romano, Marco; Ricci, Vittorio; MacDonald, Thomas T.; Pallone, Francesco; Monteleone, Giovanni.

In: Journal of Immunology, Vol. 178, No. 9, 01.05.2007, p. 5957-5965.

Research output: Contribution to journalArticle

Caruso, R, Fina, D, Peluso, I, Fantini, MC, Tosti, C, Blanco, GDV, Paoluzi, OA, Caprioli, F, Andrei, F, Stolfi, C, Romano, M, Ricci, V, MacDonald, TT, Pallone, F & Monteleone, G 2007, 'IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis', Journal of Immunology, vol. 178, no. 9, pp. 5957-5965.
Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Blanco GDV et al. IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis. Journal of Immunology. 2007 May 1;178(9):5957-5965.
Caruso, Roberta ; Fina, Daniele ; Peluso, Ilaria ; Fantini, Massimo Claudio ; Tosti, Claudio ; Blanco, Giovanna Del Vecchio ; Paoluzi, Omero Alessandro ; Caprioli, Flavio ; Andrei, Fabio ; Stolfi, Carmine ; Romano, Marco ; Ricci, Vittorio ; MacDonald, Thomas T. ; Pallone, Francesco ; Monteleone, Giovanni. / IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis. In: Journal of Immunology. 2007 ; Vol. 178, No. 9. pp. 5957-5965.
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abstract = "Helicobacter pylori (Hp) infection is associated with gastric inflammation and ulceration. The pathways of tissue damage in Hp-infected subjects are complex, but evidence indicates that T cell-derived cytokines enhance the synthesis of matrix metalloproteinases (MMP) that contribute to mucosal ulceration and epithelial damage. In this study, we have examined the role of the T cell cytokine IL-21 in Hp-infected gastric mucosa and evaluated whether IL-21 regulates MMP production by gastric epithelial cells. We show that IL-21 is constitutively expressed in gastric mucosa and is more abundant in biopsy specimens and purified mucosal CD3+ T cells from Hp-infected patients compared with normal patients and disease controls. We also demonstrate that IL-21R is expressed by primary gastric epithelial cells, as well as by the gastric epithelial cell lines AGS and MKN28. Consistently, AGS cells respond to IL-21 by increasing production of MMP-2 and MMP-9, but not MMP-1, MMP-3, MMP-7, or tissue inhibitors of MMP. Analysis of signaling pathways leading to MMP production reveals that IL-21 enhances NF-κB but not MAPK activation, and inhibition of NF-κB activation reduces IL-21-induced MMP-2 and MMP-9 production. Finally, we show that treatment of Hp-infected gastric explants with anti-IL-21 reduces epithelial cell-derived MMP-2 and MMP-9 production. These data indicate that IL-21 is overexpressed in Hp-infected gastric mucosa where it could contribute to increased epithelial gelatinase production.",
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AU - Tosti, Claudio

AU - Blanco, Giovanna Del Vecchio

AU - Paoluzi, Omero Alessandro

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AU - Andrei, Fabio

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AU - Romano, Marco

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AU - MacDonald, Thomas T.

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