IL-22 participates in an innate anti-HIV-1 host-resistance network through acute-phase protein induction

Dorothée Missé, Hans Yssel, Daria Trabattoni, Christelle Oblet, Sergio Lo Caputo, Francesco Mazzotta, Jérome Pène, Jean Paul Gonzalez, Mario Clerici, Francisco Veas

Research output: Contribution to journalArticle

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Abstract

Certain individuals are resistant to HIV-1 infection, despite repeated exposure to the virus. Although protection against HIV-1 infection in a small proportion of Caucasian individuals is associated with mutant alleles of the CCR5 HIV-1 coreceptor, the molecular mechanism underlying resistance in repeatedly HIV-1-exposed, uninfected individuals (EU) is unclear. In this study, we performed complementary transcriptome and proteome analyses on peripheral blood T cells, and plasma or serum from EU, their HIV-1-infected sexual partners, and healthy controls, all expressing wild-type CCR5. We report that activated T cells from EU overproduce several proteins involved in the innate immunity response, principally those including high levels of peroxiredoxin II, a NK-enhancing factor possessing strong anti-HIV activity, and IL-22, a cytokine involved in the production of acute-phase proteins such as the acute-phase serrai amyloid A (A-SAA). Cell supernatants and serum levels of these proteins were upregulated in EU. Moreover, a specific biomarker for EU detected in plasma was identified as an 8.6-kDa A-SAA cleavage product. Incubation of in vitro-generated myeloid immature dendritic cells with A-SAA resulted in CCR5 phosphorylation, down-regulation of CCR5 expression, and strongly decreased susceptibility of these cells to in vitro infection with a primary HIV-1 isolate. Taken together, these results suggest new correlates of EU protection and identify a cascade involving IL-22 and the acute phase protein pathway that is associated with innate host resistance to HIV infection.

Original languageEnglish
Pages (from-to)407-415
Number of pages9
JournalJournal of Immunology
Volume178
Issue number1
Publication statusPublished - Jan 1 2007

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Acute-Phase Proteins
HIV-1
Amyloid
HIV Infections
Peroxiredoxins
T-Lymphocytes
Sexual Partners
Gene Expression Profiling
Proteome
Innate Immunity
Dendritic Cells
interleukin-22
Blood Proteins
Blood Cells
Down-Regulation
Biomarkers
Alleles
Phosphorylation
HIV
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Missé, D., Yssel, H., Trabattoni, D., Oblet, C., Lo Caputo, S., Mazzotta, F., ... Veas, F. (2007). IL-22 participates in an innate anti-HIV-1 host-resistance network through acute-phase protein induction. Journal of Immunology, 178(1), 407-415.

IL-22 participates in an innate anti-HIV-1 host-resistance network through acute-phase protein induction. / Missé, Dorothée; Yssel, Hans; Trabattoni, Daria; Oblet, Christelle; Lo Caputo, Sergio; Mazzotta, Francesco; Pène, Jérome; Gonzalez, Jean Paul; Clerici, Mario; Veas, Francisco.

In: Journal of Immunology, Vol. 178, No. 1, 01.01.2007, p. 407-415.

Research output: Contribution to journalArticle

Missé, D, Yssel, H, Trabattoni, D, Oblet, C, Lo Caputo, S, Mazzotta, F, Pène, J, Gonzalez, JP, Clerici, M & Veas, F 2007, 'IL-22 participates in an innate anti-HIV-1 host-resistance network through acute-phase protein induction', Journal of Immunology, vol. 178, no. 1, pp. 407-415.
Missé D, Yssel H, Trabattoni D, Oblet C, Lo Caputo S, Mazzotta F et al. IL-22 participates in an innate anti-HIV-1 host-resistance network through acute-phase protein induction. Journal of Immunology. 2007 Jan 1;178(1):407-415.
Missé, Dorothée ; Yssel, Hans ; Trabattoni, Daria ; Oblet, Christelle ; Lo Caputo, Sergio ; Mazzotta, Francesco ; Pène, Jérome ; Gonzalez, Jean Paul ; Clerici, Mario ; Veas, Francisco. / IL-22 participates in an innate anti-HIV-1 host-resistance network through acute-phase protein induction. In: Journal of Immunology. 2007 ; Vol. 178, No. 1. pp. 407-415.
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