IL-4 inhibits binding and cytotoxicity of NK cells to vascular endothelium

Carla Paganin, Christian Matteucci, Salvatore Cenzuales, Alberto Mantovani, Paola Allavena

Research output: Contribution to journalArticle

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Abstract

We investigated the influence of IL-4 on the interaction between Natural Killer (NK) cells and vascular endothelial cells (EC). Pretreatment of NK cells with IL-4 inhibited the adhesion of NK cells on resting or IL-1-activated EC. The inhibitory action of IL-4 was observed on both unstimulated NK cells as well as on cells concomitantly activated with IL-2 or with phorbol ester. IL-4 also inhibited the cytotoxicity of IL-2 activated NK cells on EC. Binding of NK cells to vascular EC involves the LFA1/ICAM-1 and 2, and VLA-4/VCAM-1 adhesion pathway. Anti-CD18 mAb had a lower inhibitory effect in IL-4-treated NK cells compared to control. The levels of inhibition with resting vs IL-1-activated EC, as well as antibody blocking experiments, suggested that IL-4 exerts an inhibitory influence predominantly on the β2-integrin (CD18)-dependent adhesion pathway; nevertheless IL-4 did not affect the expression of CD18/CD11a and VLA-4 on the NK cell surface, as assessed by flow cytometry. NK cells are potent producers of IFN-γ and there is evidence that they play a role in orienting immunity to the TH1-type responses. The inhibition by IL-4 of NK cell binding to EC and subsequent recruitment and activation may thus contribute to development of TH2 responses.

Original languageEnglish
Pages (from-to)135-140
Number of pages6
JournalCytokine
Volume6
Issue number2
DOIs
Publication statusPublished - 1994

Fingerprint

Vascular Endothelium
Cytotoxicity
Natural Killer Cells
Interleukin-4
Endothelial cells
Endothelial Cells
Integrin alpha4beta1
Adhesion
Interleukin-1
Interleukin-2
Blocking Antibodies
Flow cytometry
Vascular Cell Adhesion Molecule-1
Phorbol Esters
Intercellular Adhesion Molecule-1
Integrins
Chemical activation
Immunity
Flow Cytometry

Keywords

  • Adhesion molecules
  • Cytokines
  • NK cells
  • Vascular endothelium

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

IL-4 inhibits binding and cytotoxicity of NK cells to vascular endothelium. / Paganin, Carla; Matteucci, Christian; Cenzuales, Salvatore; Mantovani, Alberto; Allavena, Paola.

In: Cytokine, Vol. 6, No. 2, 1994, p. 135-140.

Research output: Contribution to journalArticle

Paganin, Carla ; Matteucci, Christian ; Cenzuales, Salvatore ; Mantovani, Alberto ; Allavena, Paola. / IL-4 inhibits binding and cytotoxicity of NK cells to vascular endothelium. In: Cytokine. 1994 ; Vol. 6, No. 2. pp. 135-140.
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