IL-8 enhances antibody-dependent cellular cytotoxicity in human neutrophils

E. Reali, S. Spisani, R. Gavioli, F. Lanza, S. Moretti, S. Traniello

Research output: Contribution to journalArticle

Abstract

Human neutrophils are able to kill in vitro colorectal carcinoma cell line SW11-16 coated with mAb 17-1A, but they are not cytotoxic towards a non-immunized tumour target. Neutrophil exposure to the inflammatory cytokine, IL-8, produces a significant increase in antibody-dependent cellular cytotoxicity, which is related to IL-8 concentration. Oxyradical production is one of the lytic mechanisms used by phagocytes, and IL-8 is shown to activate this function, which does not occur if neutrophils are pretreated with the protein kinase C inhibitor, staurosporine, but is increased by R9022, a dyacylglycerol kinase inhibitor. The IL-8 effect is mediated by protein kinase C, which is potentiated by the calcium nux induced by the interaction between antibody coating tumour target and FcγRIII on effector cells, as previously demonstrated. Data suggest a possible new role for IL-8 in tumour surveillance.

Original languageEnglish
Pages (from-to)234-238
Number of pages5
JournalImmunology and Cell Biology
Volume73
Issue number3
Publication statusPublished - 1995

Keywords

  • Colorectal carcinoma cell line
  • Cytotoxicity
  • Interleukin-8
  • Neutrophil
  • Respiratory burst

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Immunology

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  • Cite this

    Reali, E., Spisani, S., Gavioli, R., Lanza, F., Moretti, S., & Traniello, S. (1995). IL-8 enhances antibody-dependent cellular cytotoxicity in human neutrophils. Immunology and Cell Biology, 73(3), 234-238.