TY - JOUR
T1 - ImageStream promyelocytic leukemia protein immunolocalization
T2 - In search of promyelocytic leukemia cells
AU - Mirabelli, Peppino
AU - Scalia, Giulia
AU - Pascariello, Caterina
AU - D'Alessio, Francesca
AU - Mariotti, Elisabetta
AU - Noto, Rosa Di
AU - George, Thaddeus C.
AU - Kong, Raymond
AU - Venkatachalam, Vidya
AU - Basiji, David
AU - Vecchio, Luigi Del
PY - 2012/3
Y1 - 2012/3
N2 - Acute promyelocytic leukemia (APL) is a hematological emergency in which a rapid diagnosis is essential for early administration of appropriate therapy, including all-trans retinoic acid before the onset of fatal coagulopathy. Currently, the following methodologies are widely used for rapid initial diagnosis of APL: 1) identification of hypergranular leukemic promyelocytes by using classical morphology; 2) identification of cells with diffuse promyelocytic leukemia (PML) protein distribution by immunofluorescence microscopy; 3) evidence of aberrant promyelocyte surface immunophenotype by conventional flow cytometry (FCM). Here, we show a method for immunofluorescent detection of PML localization using ImageStream FCM. This technique provides objective per-cell quantitative image analysis for statistically large sample sizes, enabling precise and operator-independent PML pattern recognition even in electronic and real dilution experiments up to 10% of APL cellular presence. Therefore, we evidence that this method could be helpful for rapid and objective initial diagnosis and the prompt initiation of APL treatment.
AB - Acute promyelocytic leukemia (APL) is a hematological emergency in which a rapid diagnosis is essential for early administration of appropriate therapy, including all-trans retinoic acid before the onset of fatal coagulopathy. Currently, the following methodologies are widely used for rapid initial diagnosis of APL: 1) identification of hypergranular leukemic promyelocytes by using classical morphology; 2) identification of cells with diffuse promyelocytic leukemia (PML) protein distribution by immunofluorescence microscopy; 3) evidence of aberrant promyelocyte surface immunophenotype by conventional flow cytometry (FCM). Here, we show a method for immunofluorescent detection of PML localization using ImageStream FCM. This technique provides objective per-cell quantitative image analysis for statistically large sample sizes, enabling precise and operator-independent PML pattern recognition even in electronic and real dilution experiments up to 10% of APL cellular presence. Therefore, we evidence that this method could be helpful for rapid and objective initial diagnosis and the prompt initiation of APL treatment.
KW - Acute promyelocytic leukemia
KW - All-trans retinoic acid
KW - Flow cytometry
KW - ImageStream
KW - PML gene
UR - http://www.scopus.com/inward/record.url?scp=84857369761&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84857369761&partnerID=8YFLogxK
U2 - 10.1002/cyto.a.22013
DO - 10.1002/cyto.a.22013
M3 - Article
C2 - 22266986
AN - SCOPUS:84857369761
VL - 81 A
SP - 232
EP - 237
JO - Cytometry. Part A : the journal of the International Society for Analytical Cytology
JF - Cytometry. Part A : the journal of the International Society for Analytical Cytology
SN - 1552-4922
IS - 3
ER -