Imaging single nanoparticle interactions with human lung cells using fast ion conductance microscopy

Pavel Novak, Andrew Shevchuk, Pakatip Ruenraroengsak, Michele Miragoli, Andrew J. Thorley, David Klenerman, Max J. Lab, Teresa D. Tetley, Julia Gorelik, Yuri E. Korchev

Research output: Contribution to journalArticle

Abstract

Experimental data on dynamic interactions between individual nanoparticles and membrane processes at nanoscale, essential for biomedical applications of nanoparticles, remain scarce due to limitations of imaging techniques. We were able to follow single 200 nm carboxyl-modified particles interacting with identified membrane structures at the rate of 15 s/frame using a scanning ion conductance microscope modified for simultaneous high-speed topographical and fluorescence imaging. The imaging approach demonstrated here opens a new window into the complexity of nanoparticle-cell interactions.

Original languageEnglish
Pages (from-to)1202-1207
Number of pages6
JournalNano Letters
Volume14
Issue number3
DOIs
Publication statusPublished - Mar 12 2014

Keywords

  • actin
  • alveolar epithelial cells
  • clathrin
  • Nanoparticles
  • scanning ion conductance microscopy

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

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  • Cite this

    Novak, P., Shevchuk, A., Ruenraroengsak, P., Miragoli, M., Thorley, A. J., Klenerman, D., Lab, M. J., Tetley, T. D., Gorelik, J., & Korchev, Y. E. (2014). Imaging single nanoparticle interactions with human lung cells using fast ion conductance microscopy. Nano Letters, 14(3), 1202-1207. https://doi.org/10.1021/nl404068p