Imbalance of osteoclastogenesis-regulating factors in patients with celiac disease

Anna Taranta, Dario Fortunati, Maurizio Longo, Nadia Rucci, Enzo Iacomino, Ferdinando Aliberti, Enzo Facciuto, Silvia Migliaccio, Maria Teresa Bardella, Antonella Dubini, Maria Orietta Borghi, Silvia Saraifoger, Anna Teti, Maria Luisa Bianchi

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Abstract

Celiac disease is an autoimmune disorder characterized by atrophy of the intestine villi triggered by ingestion of gluten in genetically susceptible individuals. The association between celiac disease and low BMD has been recognized, but the mechanisms of disturbance are poorly understood. We show imbalance of cytokines relevant to bone metabolism in celiac patients' sera and the direct effect of these sera on in vitro bone cell activity. Introduction: Celiac disease is associated with mineral metabolism derangement and low BMD. We investigated whether imbalance of serum factors in celiac patients could affect human bone cell activity in vitro. Materials and Methods: We studied two groups of celiac patients - one on a gluten-free diet and another before the diet - both with decreased bone mass. Patients were investigated for bone turnover markers, and their sera were used for culturing bone cells from healthy donors and evaluate changes in cell activity. Results: The N-terminal telopeptide of procollagen type I and interleukin (IL)-6 were higher than normal in patients not on the gluten-free diet. IL-1β and TNF-α/β were normal in all patients. IL-12 was reduced in all patients, whereas IL-18 was reduced only in patients on the diet. The RANKL/osteoprotegerin (OPG) ratio was increased in patients not on the gluten-free diet. Persistently increased osteoclast numbers were obtained from peripheral blood mononuclear cells of healthy donors on incubation with sera of patients not on the gluten-free diet versus control sera and sera from patients on the diet. In human osteoblasts from healthy individuals, IL-18 was reduced on incubation with sera from all patients, whereas OPG expression was lower when sera from patients not on the diet were used. Proliferation, alkaline phosphatase, and nodule mineralization were increased in osteoblast cultures containing sera from all celiac patients, either on or not on the gluten-free diet. Conclusions: We conclude that bone loss in celiac disease might also be caused by a cytokine imbalance directly affecting osteoclastogenesis and osteoblast activity.

Original languageEnglish
Pages (from-to)1112-1121
Number of pages10
JournalJournal of Bone and Mineral Research
Volume19
Issue number7
DOIs
Publication statusPublished - Jul 2004

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Celiac Disease
Osteogenesis
Gluten-Free Diet
Serum
Bone and Bones
Abdomen
Osteoblasts
Diet
Osteoprotegerin
Interleukin-18
Tissue Donors
Cytokines
Glutens
Bone Remodeling
Osteoclasts
Interleukin-12
Collagen Type I
Interleukin-1
Atrophy
Intestines

Keywords

  • Bone resorption
  • Celiac disease
  • Osteoblast
  • Osteoclast
  • Osteoporosis

ASJC Scopus subject areas

  • Surgery

Cite this

Imbalance of osteoclastogenesis-regulating factors in patients with celiac disease. / Taranta, Anna; Fortunati, Dario; Longo, Maurizio; Rucci, Nadia; Iacomino, Enzo; Aliberti, Ferdinando; Facciuto, Enzo; Migliaccio, Silvia; Bardella, Maria Teresa; Dubini, Antonella; Borghi, Maria Orietta; Saraifoger, Silvia; Teti, Anna; Bianchi, Maria Luisa.

In: Journal of Bone and Mineral Research, Vol. 19, No. 7, 07.2004, p. 1112-1121.

Research output: Contribution to journalArticle

Taranta, A, Fortunati, D, Longo, M, Rucci, N, Iacomino, E, Aliberti, F, Facciuto, E, Migliaccio, S, Bardella, MT, Dubini, A, Borghi, MO, Saraifoger, S, Teti, A & Bianchi, ML 2004, 'Imbalance of osteoclastogenesis-regulating factors in patients with celiac disease', Journal of Bone and Mineral Research, vol. 19, no. 7, pp. 1112-1121. https://doi.org/10.1359/JBMR.040319
Taranta A, Fortunati D, Longo M, Rucci N, Iacomino E, Aliberti F et al. Imbalance of osteoclastogenesis-regulating factors in patients with celiac disease. Journal of Bone and Mineral Research. 2004 Jul;19(7):1112-1121. https://doi.org/10.1359/JBMR.040319
Taranta, Anna ; Fortunati, Dario ; Longo, Maurizio ; Rucci, Nadia ; Iacomino, Enzo ; Aliberti, Ferdinando ; Facciuto, Enzo ; Migliaccio, Silvia ; Bardella, Maria Teresa ; Dubini, Antonella ; Borghi, Maria Orietta ; Saraifoger, Silvia ; Teti, Anna ; Bianchi, Maria Luisa. / Imbalance of osteoclastogenesis-regulating factors in patients with celiac disease. In: Journal of Bone and Mineral Research. 2004 ; Vol. 19, No. 7. pp. 1112-1121.
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AU - Iacomino, Enzo

AU - Aliberti, Ferdinando

AU - Facciuto, Enzo

AU - Migliaccio, Silvia

AU - Bardella, Maria Teresa

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KW - Celiac disease

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