To determine if whole-body irradiation with ultraviolet light can induce immune complex formation both in skin and in circulation, NZB W F1 mice with high serum titers of anti-nuclear antibodies (ANA) and anti-DNA antibodies were submitted to prolonged uv irradiation. Skin biopsies and serum samples collected at different times after uv exposure showed a high incidence of ANA binding to epidermal cell nuclei and decreased serum DNA binding activity, suggesting that circulating DNA-anti DNA immune complexes had formed. This interpretation was supported by the observation that treatment of the postirradiation sera with DNase partially restored their DNA binding activity.
ASJC Scopus subject areas
- Immunology and Allergy
- Pathology and Forensic Medicine