Immune complexes and IgG-Fc receptors on human platelets in essential mixed cryoglobulinemia

Sebastiano Garelli, Pier Luigi Meroni, Giuseppina Musmanno, Wilma Barcellini, Fulvio Invernizzi

Research output: Contribution to journalArticlepeer-review

Abstract

An enzyme-linked immunosorbent assay (ELISA) using wells coated with normal platelets has been tested in order to quantitate cell-bound immune complexes (ICs) by measuring the uptake of alkaline phosphatase-conjugated antibodies to IgG in the immune complexes. All patients with essential mixed cryoglobulinemia had levels of serum IgG ICs above control values; a poor accordance was found between levels of ICs detected by this method and those obtained using the conglutinin method. The presence of these complexes was correlated with human platelet aggregation: platelets aggregated in vitro when cryoglobulins with higher IgG/IgM ratio were present. This type of ICs could be critical also for platelet activation and aggregation in vivo.

Original languageEnglish
Pages (from-to)289-294
Number of pages6
JournalLa Ricerca in Clinica e in Laboratorio
Volume16
Issue number2
DOIs
Publication statusPublished - Apr 1986

Keywords

  • Essential mixed cryoglobulinemia
  • IgG-Fc receptors
  • Immune complexes
  • Immunoglobulins
  • Platelets

ASJC Scopus subject areas

  • Clinical Biochemistry

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