Immune complexes and IgG-Fc receptors on human platelets in essential mixed cryoglobulinemia

Sebastiano Garelli; Pier Luigi Meroni; Giuseppina Musmanno; Wilma Barcellini; Fulvio Invernizzi

doi:10.1007/BF02909352

Immune complexes and IgG-Fc receptors on human platelets in essential mixed cryoglobulinemia

Sebastiano Garelli, Pier Luigi Meroni, Giuseppina Musmanno, Wilma Barcellini, Fulvio Invernizzi

Research output: Contribution to journal › Article › peer-review

Abstract

An enzyme-linked immunosorbent assay (ELISA) using wells coated with normal platelets has been tested in order to quantitate cell-bound immune complexes (ICs) by measuring the uptake of alkaline phosphatase-conjugated antibodies to IgG in the immune complexes. All patients with essential mixed cryoglobulinemia had levels of serum IgG ICs above control values; a poor accordance was found between levels of ICs detected by this method and those obtained using the conglutinin method. The presence of these complexes was correlated with human platelet aggregation: platelets aggregated in vitro when cryoglobulins with higher IgG/IgM ratio were present. This type of ICs could be critical also for platelet activation and aggregation in vivo.

Original language	English
Pages (from-to)	289-294
Number of pages	6
Journal	La Ricerca in Clinica e in Laboratorio
Volume	16
Issue number	2
DOIs	https://doi.org/10.1007/BF02909352
Publication status	Published - Apr 1986

Keywords

Essential mixed cryoglobulinemia
IgG-Fc receptors
Immune complexes
Immunoglobulins
Platelets

ASJC Scopus subject areas

Clinical Biochemistry

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10.1007/BF02909352

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title = "Immune complexes and IgG-Fc receptors on human platelets in essential mixed cryoglobulinemia",

abstract = "An enzyme-linked immunosorbent assay (ELISA) using wells coated with normal platelets has been tested in order to quantitate cell-bound immune complexes (ICs) by measuring the uptake of alkaline phosphatase-conjugated antibodies to IgG in the immune complexes. All patients with essential mixed cryoglobulinemia had levels of serum IgG ICs above control values; a poor accordance was found between levels of ICs detected by this method and those obtained using the conglutinin method. The presence of these complexes was correlated with human platelet aggregation: platelets aggregated in vitro when cryoglobulins with higher IgG/IgM ratio were present. This type of ICs could be critical also for platelet activation and aggregation in vivo.",

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AU - Garelli, Sebastiano

AU - Meroni, Pier Luigi

AU - Musmanno, Giuseppina

AU - Barcellini, Wilma

AU - Invernizzi, Fulvio

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