Abstract
An enzyme-linked immunosorbent assay (ELISA) using wells coated with normal platelets has been tested in order to quantitate cell-bound immune complexes (ICs) by measuring the uptake of alkaline phosphatase-conjugated antibodies to IgG in the immune complexes. All patients with essential mixed cryoglobulinemia had levels of serum IgG ICs above control values; a poor accordance was found between levels of ICs detected by this method and those obtained using the conglutinin method. The presence of these complexes was correlated with human platelet aggregation: platelets aggregated in vitro when cryoglobulins with higher IgG/IgM ratio were present. This type of ICs could be critical also for platelet activation and aggregation in vivo.
Original language | English |
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Pages (from-to) | 289-294 |
Number of pages | 6 |
Journal | La Ricerca in Clinica e in Laboratorio |
Volume | 16 |
Issue number | 2 |
DOIs | |
Publication status | Published - Apr 1986 |
Keywords
- Essential mixed cryoglobulinemia
- IgG-Fc receptors
- Immune complexes
- Immunoglobulins
- Platelets
ASJC Scopus subject areas
- Clinical Biochemistry