Immune complexes of IgG1 and IgG3 subclasses in human breast cancer sera detected by monoclonal antibodies in an indirect immunoenzymatic assay

Ciro Manzo, Armando Bianchin, Giuseppe Pirozzi, Giuseppe Totaro

Research output: Contribution to journalArticlepeer-review

Abstract

Circulating immune complexes (CIC) of the IgG class were detected in sera from 37 (35.58%) out of 104 of the preoperated breast cancer patients studied using a Clq solid-phase enzymatic assay. Sera of positive patients were then tested to identify the IgG subclasses which are part of the complexes with the aim of showing a better correlation between CIC and disease evolution. IgG1- and IgG3-CIC were indirectly detected by the above test using monoclonal antibodies against the mentioned subclasses. Curves obtained with aggregated IgG1 and/or IgG3 showed good sensitivity and specificity of the test. Complexes containing both IgG1 and IgG3 were present in sera from the majority of patients; however, the relative amounts of these subclasses varied from patient to patient.

Original languageEnglish
Pages (from-to)395-399
Number of pages5
JournalOncology
Volume40
Issue number6
DOIs
Publication statusPublished - 1983

Keywords

  • Breasl cancer
  • IgG subclasses
  • Immune complexes
  • Immunoregulation
  • Monoclonal antibodies

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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