Immune electron microscopy of hepatitis δ-antigen in hepatocytes

T. Kojima, F. Callea, J. Desmyter, V. J. Desmet

Research output: Contribution to journalArticle

Abstract

Immune electron microscopic examination of hepatitis delta (δ)-antigen (HDAg) was carried out on liver biopsies from seven patients with chronic type B hepatitis and δ-infection. HDAg was mainly found in the nucleus, but in some scattered hepatocytes it was found in the cytoplasm also, either in association with ribosomes or in the hyaloplasm. The association of HDAg with ribosomes may be the result of translation from δ-messenger RNA on the same ribosomes. In the nucleus, δ-antigen was present in the chromatin area as irregular granular structures, and there was staining of aggregates of 20 to 30-nm particulate structures, similar to those first described in non-A, non-B hepatitis. However, similar particulate structures were also present in HDAg negative nuclei. The δ-particle which have been described in blood could not be identified in hepatocytes.

Original languageEnglish
Pages (from-to)217-225
Number of pages9
JournalLaboratory Investigation
Volume55
Issue number2
Publication statusPublished - 1986

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Kojima, T., Callea, F., Desmyter, J., & Desmet, V. J. (1986). Immune electron microscopy of hepatitis δ-antigen in hepatocytes. Laboratory Investigation, 55(2), 217-225.