Immune reaction against the cytoskeleton in coeliac disease

M. G. Clemente, M. P. Musu, F. Frau, G. Brusco, G. Sole, G. R. Corazza, S. De Virgiliis

Research output: Contribution to journalArticlepeer-review

Abstract

Background - The cytoskeleton actin network of intestinal microvilli has been found to be rapidly impaired after gluten challenge in coeliac disease (CD). The aim of this study was to investigate the presence of an immune reaction towards cytoskeleton structures such as actin filaments in CD. Methods - Eighty three antiendomysial antibody positive CD patients (52 children and 31 adults) were studied at our outpatient clinics from 1996 to 1998 using indirect immunofluorescence, ELISA, and western blotting for antiactin (AAA) and antitissue transglutaminase (TGA) antibodies before and after a gluten free diet (GFD). Sixteen patients with smooth muscle antibody positive autoimmune hepatitis, 21 with inflammatory bowel diseases, seven with small bowel bacterial overgrowth, and 60 healthy subjects were studied as controls. Results - Fifty nine of 83 CD patients (28/31 adults (90.3%); 31/52 children (59.6%)) were positive for IgA and/or IgG AAA. Seventy seven (92.7%) were positive for IgA TGA. IgA AAA were strongly correlated with more severe degrees of intestinal villous atrophy (p

Original languageEnglish
Pages (from-to)520-526
Number of pages7
JournalGut
Volume47
Issue number4
DOIs
Publication statusPublished - 2000

Keywords

  • Actin
  • Autoantibody
  • Coeliac disease
  • Cytoskeleton
  • Intestinal villous atrophy
  • Smooth muscle

ASJC Scopus subject areas

  • Gastroenterology

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