Abstract
A plasmid DNA containing two different expression cassettes was prepared to independently drive antigen 85B (85B) of Mycobacterium tuberculosis and HIV-Tat in C57BL/6 mice. In vivo expression of the plasmid was demonstrated by efficient transcription of 85B and Tat mRNAs in mouse fibroblasts. DNA-85B or DNA-(85B-Tat) were immunogenic and protected mice to the same extent against M. tuberculosis infection, with a decrease in the numbers of CFU lung-1 in comparison with nonimmunized animals down to levels (0.64 log10 CFU) not significantly different from protection conferred by bacillus Calmette-Guérin vaccine (0.97 log10 CFU decrease). Multipromoter plasmids, which permit the reduction of the total amount of DNA injected, can be useful for DNA vaccination against tuberculosis.
Original language | English |
---|---|
Pages (from-to) | 210-215 |
Number of pages | 6 |
Journal | FEMS Microbiology Letters |
Volume | 262 |
Issue number | 2 |
DOIs | |
Publication status | Published - Sep 2006 |
Keywords
- 85B antigen
- DNA vaccines
- HIV-Tat
- Immune responses
- Mice protection
- Tuberculosis
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Applied Microbiology and Biotechnology
- Microbiology