Immune response and protection by DNA vaccines expressing antigen 85B of Mycobacterium tuberculosis

Manuela Pardini; Federico Giannoni; Carla Palma; Elisabetta Iona; Aurelio Cafaro; Lara Brunori; Monica Rinaldi; Vito Michele Fazio; Maria Elena Laguardia; Damiano Cosimo Carbonella; Mauro Magnani; Barbara Ensoli; Lanfranco Fattorini; Antonio Cassone

doi:10.1111/j.1574-6968.2006.00390.x

Immune response and protection by DNA vaccines expressing antigen 85B of Mycobacterium tuberculosis

Manuela Pardini, Federico Giannoni, Carla Palma, Elisabetta Iona, Aurelio Cafaro, Lara Brunori, Monica Rinaldi, Vito Michele Fazio, Maria Elena Laguardia, Damiano Cosimo Carbonella, Mauro Magnani, Barbara Ensoli, Lanfranco Fattorini, Antonio Cassone

IRCCS Casa Sollievo della Sofferenza

Research output: Contribution to journal › Article

Abstract

A plasmid DNA containing two different expression cassettes was prepared to independently drive antigen 85B (85B) of Mycobacterium tuberculosis and HIV-Tat in C57BL/6 mice. In vivo expression of the plasmid was demonstrated by efficient transcription of 85B and Tat mRNAs in mouse fibroblasts. DNA-85B or DNA-(85B-Tat) were immunogenic and protected mice to the same extent against M. tuberculosis infection, with a decrease in the numbers of CFU lung^-1 in comparison with nonimmunized animals down to levels (0.64 log10 CFU) not significantly different from protection conferred by bacillus Calmette-Guérin vaccine (0.97 log10 CFU decrease). Multipromoter plasmids, which permit the reduction of the total amount of DNA injected, can be useful for DNA vaccination against tuberculosis.

Original language	English
Pages (from-to)	210-215
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	262
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6968.2006.00390.x
Publication status	Published - Sep 2006

Keywords

85B antigen
DNA vaccines
HIV-Tat
Immune responses
Mice protection
Tuberculosis

ASJC Scopus subject areas

Genetics
Molecular Biology
Applied Microbiology and Biotechnology
Microbiology

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Pardini, M., Giannoni, F., Palma, C., Iona, E., Cafaro, A., Brunori, L., Rinaldi, M., Fazio, V. M., Laguardia, M. E., Carbonella, D. C., Magnani, M., Ensoli, B., Fattorini, L., & Cassone, A. (2006). Immune response and protection by DNA vaccines expressing antigen 85B of Mycobacterium tuberculosis. FEMS Microbiology Letters, 262(2), 210-215. https://doi.org/10.1111/j.1574-6968.2006.00390.x

Immune response and protection by DNA vaccines expressing antigen 85B of Mycobacterium tuberculosis. / Pardini, Manuela; Giannoni, Federico; Palma, Carla; Iona, Elisabetta; Cafaro, Aurelio; Brunori, Lara; Rinaldi, Monica; Fazio, Vito Michele; Laguardia, Maria Elena; Carbonella, Damiano Cosimo; Magnani, Mauro; Ensoli, Barbara; Fattorini, Lanfranco; Cassone, Antonio.

In: FEMS Microbiology Letters, Vol. 262, No. 2, 09.2006, p. 210-215.

Research output: Contribution to journal › Article

Pardini, M, Giannoni, F, Palma, C, Iona, E, Cafaro, A, Brunori, L, Rinaldi, M, Fazio, VM, Laguardia, ME, Carbonella, DC, Magnani, M, Ensoli, B, Fattorini, L & Cassone, A 2006, 'Immune response and protection by DNA vaccines expressing antigen 85B of Mycobacterium tuberculosis', FEMS Microbiology Letters, vol. 262, no. 2, pp. 210-215. https://doi.org/10.1111/j.1574-6968.2006.00390.x

Pardini, Manuela ; Giannoni, Federico ; Palma, Carla ; Iona, Elisabetta ; Cafaro, Aurelio ; Brunori, Lara ; Rinaldi, Monica ; Fazio, Vito Michele ; Laguardia, Maria Elena ; Carbonella, Damiano Cosimo ; Magnani, Mauro ; Ensoli, Barbara ; Fattorini, Lanfranco ; Cassone, Antonio. / Immune response and protection by DNA vaccines expressing antigen 85B of Mycobacterium tuberculosis. In: FEMS Microbiology Letters. 2006 ; Vol. 262, No. 2. pp. 210-215.

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abstract = "A plasmid DNA containing two different expression cassettes was prepared to independently drive antigen 85B (85B) of Mycobacterium tuberculosis and HIV-Tat in C57BL/6 mice. In vivo expression of the plasmid was demonstrated by efficient transcription of 85B and Tat mRNAs in mouse fibroblasts. DNA-85B or DNA-(85B-Tat) were immunogenic and protected mice to the same extent against M. tuberculosis infection, with a decrease in the numbers of CFU lung-1 in comparison with nonimmunized animals down to levels (0.64 log10 CFU) not significantly different from protection conferred by bacillus Calmette-Gu{\'e}rin vaccine (0.97 log10 CFU decrease). Multipromoter plasmids, which permit the reduction of the total amount of DNA injected, can be useful for DNA vaccination against tuberculosis.",

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AU - Brunori, Lara

AU - Rinaldi, Monica

AU - Fazio, Vito Michele

AU - Laguardia, Maria Elena

AU - Carbonella, Damiano Cosimo

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