Abstract
A plasmid DNA containing two different expression cassettes was prepared to independently drive antigen 85B (85B) of Mycobacterium tuberculosis and HIV-Tat in C57BL/6 mice. In vivo expression of the plasmid was demonstrated by efficient transcription of 85B and Tat mRNAs in mouse fibroblasts. DNA-85B or DNA-(85B-Tat) were immunogenic and protected mice to the same extent against M. tuberculosis infection, with a decrease in the numbers of CFU lung-1 in comparison with nonimmunized animals down to levels (0.64 log10 CFU) not significantly different from protection conferred by bacillus Calmette-Guérin vaccine (0.97 log10 CFU decrease). Multipromoter plasmids, which permit the reduction of the total amount of DNA injected, can be useful for DNA vaccination against tuberculosis.
| Original language | English |
|---|---|
| Pages (from-to) | 210-215 |
| Number of pages | 6 |
| Journal | FEMS Microbiology Letters |
| Volume | 262 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Sep 2006 |
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Keywords
- 85B antigen
- DNA vaccines
- HIV-Tat
- Immune responses
- Mice protection
- Tuberculosis
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Applied Microbiology and Biotechnology
- Microbiology
Cite this
Immune response and protection by DNA vaccines expressing antigen 85B of Mycobacterium tuberculosis. / Pardini, Manuela; Giannoni, Federico; Palma, Carla; Iona, Elisabetta; Cafaro, Aurelio; Brunori, Lara; Rinaldi, Monica; Fazio, Vito Michele; Laguardia, Maria Elena; Carbonella, Damiano Cosimo; Magnani, Mauro; Ensoli, Barbara; Fattorini, Lanfranco; Cassone, Antonio.
In: FEMS Microbiology Letters, Vol. 262, No. 2, 09.2006, p. 210-215.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Immune response and protection by DNA vaccines expressing antigen 85B of Mycobacterium tuberculosis
AU - Pardini, Manuela
AU - Giannoni, Federico
AU - Palma, Carla
AU - Iona, Elisabetta
AU - Cafaro, Aurelio
AU - Brunori, Lara
AU - Rinaldi, Monica
AU - Fazio, Vito Michele
AU - Laguardia, Maria Elena
AU - Carbonella, Damiano Cosimo
AU - Magnani, Mauro
AU - Ensoli, Barbara
AU - Fattorini, Lanfranco
AU - Cassone, Antonio
PY - 2006/9
Y1 - 2006/9
N2 - A plasmid DNA containing two different expression cassettes was prepared to independently drive antigen 85B (85B) of Mycobacterium tuberculosis and HIV-Tat in C57BL/6 mice. In vivo expression of the plasmid was demonstrated by efficient transcription of 85B and Tat mRNAs in mouse fibroblasts. DNA-85B or DNA-(85B-Tat) were immunogenic and protected mice to the same extent against M. tuberculosis infection, with a decrease in the numbers of CFU lung-1 in comparison with nonimmunized animals down to levels (0.64 log10 CFU) not significantly different from protection conferred by bacillus Calmette-Guérin vaccine (0.97 log10 CFU decrease). Multipromoter plasmids, which permit the reduction of the total amount of DNA injected, can be useful for DNA vaccination against tuberculosis.
AB - A plasmid DNA containing two different expression cassettes was prepared to independently drive antigen 85B (85B) of Mycobacterium tuberculosis and HIV-Tat in C57BL/6 mice. In vivo expression of the plasmid was demonstrated by efficient transcription of 85B and Tat mRNAs in mouse fibroblasts. DNA-85B or DNA-(85B-Tat) were immunogenic and protected mice to the same extent against M. tuberculosis infection, with a decrease in the numbers of CFU lung-1 in comparison with nonimmunized animals down to levels (0.64 log10 CFU) not significantly different from protection conferred by bacillus Calmette-Guérin vaccine (0.97 log10 CFU decrease). Multipromoter plasmids, which permit the reduction of the total amount of DNA injected, can be useful for DNA vaccination against tuberculosis.
KW - 85B antigen
KW - DNA vaccines
KW - HIV-Tat
KW - Immune responses
KW - Mice protection
KW - Tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=33747655832&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33747655832&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.2006.00390.x
DO - 10.1111/j.1574-6968.2006.00390.x
M3 - Article
C2 - 16923077
AN - SCOPUS:33747655832
VL - 262
SP - 210
EP - 215
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -