Immune response and protection by DNA vaccines expressing antigen 85B of Mycobacterium tuberculosis

Manuela Pardini, Federico Giannoni, Carla Palma, Elisabetta Iona, Aurelio Cafaro, Lara Brunori, Monica Rinaldi, Vito Michele Fazio, Maria Elena Laguardia, Damiano Cosimo Carbonella, Mauro Magnani, Barbara Ensoli, Lanfranco Fattorini, Antonio Cassone

Research output: Contribution to journalArticle

Abstract

A plasmid DNA containing two different expression cassettes was prepared to independently drive antigen 85B (85B) of Mycobacterium tuberculosis and HIV-Tat in C57BL/6 mice. In vivo expression of the plasmid was demonstrated by efficient transcription of 85B and Tat mRNAs in mouse fibroblasts. DNA-85B or DNA-(85B-Tat) were immunogenic and protected mice to the same extent against M. tuberculosis infection, with a decrease in the numbers of CFU lung-1 in comparison with nonimmunized animals down to levels (0.64 log10 CFU) not significantly different from protection conferred by bacillus Calmette-Guérin vaccine (0.97 log10 CFU decrease). Multipromoter plasmids, which permit the reduction of the total amount of DNA injected, can be useful for DNA vaccination against tuberculosis.

Original languageEnglish
Pages (from-to)210-215
Number of pages6
JournalFEMS Microbiology Letters
Volume262
Issue number2
DOIs
Publication statusPublished - Sep 2006

Keywords

  • 85B antigen
  • DNA vaccines
  • HIV-Tat
  • Immune responses
  • Mice protection
  • Tuberculosis

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

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  • Cite this

    Pardini, M., Giannoni, F., Palma, C., Iona, E., Cafaro, A., Brunori, L., Rinaldi, M., Fazio, V. M., Laguardia, M. E., Carbonella, D. C., Magnani, M., Ensoli, B., Fattorini, L., & Cassone, A. (2006). Immune response and protection by DNA vaccines expressing antigen 85B of Mycobacterium tuberculosis. FEMS Microbiology Letters, 262(2), 210-215. https://doi.org/10.1111/j.1574-6968.2006.00390.x