Abstract
By exploring induction and persistence of virus-specific memory CD8+ T cells in the BM of Moloney-murine sarcoma/leukemia virus-immune mice, we observed that the amount of activated CD8+CD62L- cells increased more rapidly and persisted for a longer period than in peripheral organs. Among the CD8+CD62L- subset, the few cells, specific for M-MuLV encoded antigens, expressing TCRVβ5 rearrangements increased in an explosive manner doubling the percentage of TCRVβ5+ subset so that as a final result more than 10% of CD8+ lymphocytes became potential virus-specific cytotoxic effectors. The numerical expansion of Vβ5+ cells started and persisted in the same proportion among both CD8+CD62L- and CD8+CD62L+ subsets. In these subsets the analysis of CD44 phenotype, to distinguish effector (TEM) and central (TCM) memory, evidenced a twofold increase of Vβ5+ TEM percentage and fourfold increase of Vβ5+ TCM. In parallel, the non virus-specific Vβ5- counterpart, also numerically increased due to the CD8+ expansion, was partially reduced as TEM percentage and doubled as TCM percentage. We conclude that the immune response to M-MuLV encoded antigens in BM generate not only a large number of virus-specific memory cells but also the re-shaping of the entire memory T cell repertoire.
| Original language | English |
|---|---|
| Pages (from-to) | 79-85 |
| Number of pages | 7 |
| Journal | Immunology Letters |
| Volume | 138 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jul 2011 |
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Keywords
- Bone marrow
- CD8 T cells
- T cell activation
- T cell memory
- T cell repertoire
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Immune response to Moloney-murine leukemia virus-induced antigens in bone marrow. / Biasi, Giovanni; Facchinetti, Antonella; Cappellari, Roberta; Rossi, Elisabetta; Zanovello, Paola.
In: Immunology Letters, Vol. 138, No. 1, 07.2011, p. 79-85.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immune response to Moloney-murine leukemia virus-induced antigens in bone marrow
AU - Biasi, Giovanni
AU - Facchinetti, Antonella
AU - Cappellari, Roberta
AU - Rossi, Elisabetta
AU - Zanovello, Paola
PY - 2011/7
Y1 - 2011/7
N2 - By exploring induction and persistence of virus-specific memory CD8+ T cells in the BM of Moloney-murine sarcoma/leukemia virus-immune mice, we observed that the amount of activated CD8+CD62L- cells increased more rapidly and persisted for a longer period than in peripheral organs. Among the CD8+CD62L- subset, the few cells, specific for M-MuLV encoded antigens, expressing TCRVβ5 rearrangements increased in an explosive manner doubling the percentage of TCRVβ5+ subset so that as a final result more than 10% of CD8+ lymphocytes became potential virus-specific cytotoxic effectors. The numerical expansion of Vβ5+ cells started and persisted in the same proportion among both CD8+CD62L- and CD8+CD62L+ subsets. In these subsets the analysis of CD44 phenotype, to distinguish effector (TEM) and central (TCM) memory, evidenced a twofold increase of Vβ5+ TEM percentage and fourfold increase of Vβ5+ TCM. In parallel, the non virus-specific Vβ5- counterpart, also numerically increased due to the CD8+ expansion, was partially reduced as TEM percentage and doubled as TCM percentage. We conclude that the immune response to M-MuLV encoded antigens in BM generate not only a large number of virus-specific memory cells but also the re-shaping of the entire memory T cell repertoire.
AB - By exploring induction and persistence of virus-specific memory CD8+ T cells in the BM of Moloney-murine sarcoma/leukemia virus-immune mice, we observed that the amount of activated CD8+CD62L- cells increased more rapidly and persisted for a longer period than in peripheral organs. Among the CD8+CD62L- subset, the few cells, specific for M-MuLV encoded antigens, expressing TCRVβ5 rearrangements increased in an explosive manner doubling the percentage of TCRVβ5+ subset so that as a final result more than 10% of CD8+ lymphocytes became potential virus-specific cytotoxic effectors. The numerical expansion of Vβ5+ cells started and persisted in the same proportion among both CD8+CD62L- and CD8+CD62L+ subsets. In these subsets the analysis of CD44 phenotype, to distinguish effector (TEM) and central (TCM) memory, evidenced a twofold increase of Vβ5+ TEM percentage and fourfold increase of Vβ5+ TCM. In parallel, the non virus-specific Vβ5- counterpart, also numerically increased due to the CD8+ expansion, was partially reduced as TEM percentage and doubled as TCM percentage. We conclude that the immune response to M-MuLV encoded antigens in BM generate not only a large number of virus-specific memory cells but also the re-shaping of the entire memory T cell repertoire.
KW - Bone marrow
KW - CD8 T cells
KW - T cell activation
KW - T cell memory
KW - T cell repertoire
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UR - http://www.scopus.com/inward/citedby.url?scp=79957772508&partnerID=8YFLogxK
U2 - 10.1016/j.imlet.2011.03.005
DO - 10.1016/j.imlet.2011.03.005
M3 - Article
C2 - 21453727
AN - SCOPUS:79957772508
VL - 138
SP - 79
EP - 85
JO - Immunology Letters
JF - Immunology Letters
SN - 0165-2478
IS - 1
ER -