Immunization against bovine herpesvirus-1 infection. Preliminary tests in calves with a DNA vaccine

G. Castrucci, M. Ferrari, C. Marchini, D. Salvatori, M. Provinciali, A. Tosini, S. Petrini, Q. Sardonini, M. Lo Dico, F. Frigeri, A. Amici

Research output: Contribution to journalArticlepeer-review


A bovine herpesvirus-1 (BHV-1) vaccine expressing glycoprotein D, the form with the transmembrane anchor removed, was evaluated for inducing immunity in calves. The plasmid encoding gD of BHV-1 was injected three times to nine calves, using three animals for each of the following routes: intramuscularly (i.m.), intradermally (i.d.), or intranasally (i.n.). Three additional calves were given the plasmid vector only and served as unvaccinated controls. When calves were subjected to challenge infection with BHV-1, all vaccinated calves as well as the controls developed a typical severe form of infectious bovine rhinotracheitis. However, compared to the controls, the vaccinated calves showed earlier clearance of challenge virus. Moreover, the calves given the vaccine i.m. developed neutralizing antibody to BHV-1 between 21 and 42 days following the first injection of vaccine, whereas in calves vaccinated either i.d. or i.n., as well as the controls, antibody first appeared in their sera 14 days post-challenge infection.

Original languageEnglish
Pages (from-to)171-179
Number of pages9
JournalComparative Immunology, Microbiology and Infectious Diseases
Issue number3
Publication statusPublished - May 2004


  • Bovine herpesvirus-1
  • Calves
  • DNA vaccine

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • Microbiology
  • veterinary(all)

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