Immunization of HIV-infected patients with RGP160: Modulation of anti-RGP 120 antibody spectrotype

Roberto Biselli, Lawrence D. Loomis, Valerio Del Bono, Donald S. Burke, Robert R. Redfield, Deborah L. Birx

Research output: Contribution to journalArticle

Abstract

HIV-1 infection results in progressive failure of the immune system with decline in the number and/or function of B-cell clones originally recruited in specific humoral responses. Spectrotypic analysis, done by isoelectric focusing and reverse blotting (IEF-RB), is one technique for evaluating the activity and the number of specific B-cell clones and is Actaptable to the direct measurement of antibodies to conformationally intact epitopes. The anti-HIV-1 (IIIB) rgp 120 spectrotype was measured in 30 early-stage HIV-infected volunteers undergoing vaccine therapy with recombinant gp 160 (rgp 160). Twenty-five of the patients displayed a clear oligoclonal banding pattern; seven (28%) showed the same pattern in all samples, while 18 (72%) showed changes. Ten of the latter had an increase in band intensity over the course of immunization, and eight had an increase in both band intensity and number of bands. In contrast, serum samples from eight patients receiving placebo (alum) showed no changes over a comparable period. These findings suggest that vaccine therapy with rgp 160 may be able to expand the anti-HIV-1 (LAI) gp 120 B-cell clone pool in some HIV-infected patients as well as increase antibody synthesis by established B-cell clones recruited during natural infection. These data provide further evidence that postinfection vaccination may provide an alternative strategy in the treatment of chronic viral diseases.

Original languageEnglish
Pages (from-to)1016-1024
Number of pages9
JournalJournal of Acquired Immune Deficiency Syndromes
Volume7
Issue number10
Publication statusPublished - 1994

Keywords

  • Envelope glycoprotein
  • Human immunodeficiency virus
  • Isoelectric focusing
  • Vaccine therapy

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)
  • Immunology and Allergy
  • Virology

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    Biselli, R., Loomis, L. D., Del Bono, V., Burke, D. S., Redfield, R. R., & Birx, D. L. (1994). Immunization of HIV-infected patients with RGP160: Modulation of anti-RGP 120 antibody spectrotype. Journal of Acquired Immune Deficiency Syndromes, 7(10), 1016-1024.