Immunization with HIV-1 gp41 Subunit Virosomes Induces Mucosal Antibodies Protecting Nonhuman Primates against Vaginal SHIV Challenges

Morgane Bomsel, Daniela Tudor, Anne Sophie Drillet, Annette Alfsen, Yonatan Ganor, Marie Gaëlle Roger, Nicolas Mouz, Mario Amacker, Anick Chalifour, Lorenzo Diomede, Gilles Devillier, Zhe Cong, Qiang Wei, Hong Gao, Chuan Qin, Gui Bo Yang, Rinaldo Zurbriggen, Lucia Lopalco, Sylvain Fleury

Research output: Contribution to journalArticle

Abstract

Human immunodeficiency virus (HIV)-1 is mainly transmitted mucosally during sexual intercourse. We therefore evaluated the protective efficacy of a vaccine active at mucosal sites. Macaca mulatta monkeys were immunized via both the intramuscular and intranasal routes with an HIV-1 vaccine made of gp41-subunit antigens grafted on virosomes, a safe delivery carrier approved in humans with self-adjuvant properties. Six months after 13 vaginal challenges with simian-HIV (SHIV)-SF162P3, four out of five vaccinated animals remained virus-negative, and the fifth was only transiently infected. None of the five animals seroconverted to p27. gag-SIV. In contrast, all 6 placebo-vaccinated animals became infected and seroconverted. All protected animals showed gp41-specific vaginal IgAs with HIV-1 transcytosis-blocking properties and vaginal IgGs with neutralizing and/or antibody-dependent cellular-cytotoxicity activities. In contrast, plasma IgGs totally lacked virus-neutralizing activity. The protection observed challenges the paradigm whereby circulating antiviral antibodies are required for protection against HIV-1 infection and may serve in designing a human vaccine against HIV-1-AIDS.

Original languageEnglish
Pages (from-to)269-280
Number of pages12
JournalImmunity
Volume34
Issue number2
DOIs
Publication statusPublished - Feb 25 2011

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

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    Bomsel, M., Tudor, D., Drillet, A. S., Alfsen, A., Ganor, Y., Roger, M. G., Mouz, N., Amacker, M., Chalifour, A., Diomede, L., Devillier, G., Cong, Z., Wei, Q., Gao, H., Qin, C., Yang, G. B., Zurbriggen, R., Lopalco, L., & Fleury, S. (2011). Immunization with HIV-1 gp41 Subunit Virosomes Induces Mucosal Antibodies Protecting Nonhuman Primates against Vaginal SHIV Challenges. Immunity, 34(2), 269-280. https://doi.org/10.1016/j.immuni.2011.01.015