Immunochemical characterization of human liver and heart ferritins with monoclonal antibodies

Alessandra Luzzago, Paolo Arosio, Carmelo Iacobello, Giuseppina Ruggeri, Lorenzo Capucci, Emiliana Brocchi, Franco De Simone, Daniela Gamba, Elena Gabri, Sonia Levi, Alberto Albertini

Research output: Contribution to journalArticle

Abstract

A library of 27 murine monoclonal antibodies was obtained by using human liver and heart ferritins as immunogens. The specificity of the antibodies for the two ferritins and their subunits was studied with five different methods. The antibodies elicited by the liver ferritin bound preferentially the immunogen and were specific for the L subunit. Some antibodies elicited by the heart ferritin had characteristics similar to the anti-liver antibodies, other ones bound preferentially the heart over the liver ferritin and were specific for the H subunit. Only two antibodies were able to bind both ferritins and subunits. Some anti-H and anti-L chain antibodies were used to develop and compare four types of immunoassay to quantitate isoferritins. The results indicate the heart ferritin is immunologically more heterogeneous than liver, the H and L subunits having large immunological differences with few, if any, identical epitopes; and that that the architecture of the immunoassays have a strong influence on the crossreactivity of the antibodies with the two isoferritins, probably because H and L chains are not arranged randomly in the assembled protein.

Original languageEnglish
Pages (from-to)61-71
Number of pages11
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume872
Issue number1-2
DOIs
Publication statusPublished - Jul 25 1986

Keywords

  • (Human liver, heart)
  • Isoferritin
  • Monoclonal antibody
  • Protein quaternary structure
  • Radioimmunoassay

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Structural Biology

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  • Cite this

    Luzzago, A., Arosio, P., Iacobello, C., Ruggeri, G., Capucci, L., Brocchi, E., De Simone, F., Gamba, D., Gabri, E., Levi, S., & Albertini, A. (1986). Immunochemical characterization of human liver and heart ferritins with monoclonal antibodies. Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, 872(1-2), 61-71. https://doi.org/10.1016/0167-4838(86)90147-0