Immunochemical determination of lonidamine in rat tissues and blood serum

G. Citro, R. Galati, A. Verdina, S. Castiglione, B. Silvestrini, A. Caputo, A. Floridi

Research output: Contribution to journalArticle

Abstract

Lonidamine (LND) is an antitumor drug which interferes selectively with the energy metabolism of neoplastic cell. Because of its physicochemical properties, LND determination with conventional methods, i.e. high performance liquid chromatography and spectrofluorimetry, gives rise to several problems: LND is a lipotropic drug which becomes intimately associated with biological membranes so that it is impossible to extract all the drug bound, thus leading to an underestimation of the LND content in cells and tissues. These difficulties can be overcome by the immunoenzymatic method described here. The assay is simple, rapid, practical, highly sensitive (2-5 ng/ml) and particularly suitable for the analysis of multiple samples (twelve samples in triplicate for each plate). There is, moreover, a great improvement in data reproducibility.

Original languageEnglish
Pages (from-to)811-815
Number of pages5
JournalAnticancer Research
Volume9
Issue number3
Publication statusPublished - 1989

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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