Immunocompetence of human microvascular brain endothelial cells: Cytokine regulation of IL-1β, MCP-1, IL-10, sICAM-1 and sVCAM-1

S. Frigerio, M. Gelati, E. Ciusani, E. Corsini, A. Dufour, G. Massa, A. Salmaggi

Research output: Contribution to journalArticle

Abstract

Endothelia from the brains of four patients undergoing neurosurgery, including one multiple sclerosis (MS) patient, were studied in vitro to determine cytokine and chemokine production; the release of soluble adhesion molecules was also investigated. The same procedure was repeated on human umbilical vein endothelial cells (HUVECs) in order to detect possible district-specific differences. After isolation, the endothelium was cultured and stimulated with γ-interferon (IFN), tumour necrosis factor alpha (TNF-α) and LPS. The results showed that brain endothelium, in our experimental conditions, does not produce interleukin (IL)-10 and produces lower amounts of IL-1β and soluble intercellular adhesion molecule-1 (sICAM-1) than HUVECs do; no differences were detected in soluble vascular cell adhesion molecule-1 (sVCAM-1) production. MCP-1 mRNA was detected both without and after stimulation with TNF-α and γ-IFN in HUVECs and MS human brain endothelial cells (HBECs), while in non-MS-HBECs it was found only after γ-IFN stimulation.

Original languageEnglish
Pages (from-to)727-730
Number of pages4
JournalJournal of Neurology
Volume245
Issue number11
DOIs
Publication statusPublished - 1998

Fingerprint

Immunocompetence
Vascular Cell Adhesion Molecule-1
Intercellular Adhesion Molecule-1
Interleukin-1
Interleukin-10
Human Umbilical Vein Endothelial Cells
Endothelial Cells
Cytokines
Endothelium
Brain
Interferons
Multiple Sclerosis
Tumor Necrosis Factor-alpha
Neurosurgery
Sclerosis
Chemokines
Interferon-alpha
Messenger RNA

Keywords

  • Brain endothelium
  • IL-1 beta
  • IL-10
  • MCP-1

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

Cite this

Immunocompetence of human microvascular brain endothelial cells : Cytokine regulation of IL-1β, MCP-1, IL-10, sICAM-1 and sVCAM-1. / Frigerio, S.; Gelati, M.; Ciusani, E.; Corsini, E.; Dufour, A.; Massa, G.; Salmaggi, A.

In: Journal of Neurology, Vol. 245, No. 11, 1998, p. 727-730.

Research output: Contribution to journalArticle

@article{5ebde627cc4e46049f22f586fc330e9e,
title = "Immunocompetence of human microvascular brain endothelial cells: Cytokine regulation of IL-1β, MCP-1, IL-10, sICAM-1 and sVCAM-1",
abstract = "Endothelia from the brains of four patients undergoing neurosurgery, including one multiple sclerosis (MS) patient, were studied in vitro to determine cytokine and chemokine production; the release of soluble adhesion molecules was also investigated. The same procedure was repeated on human umbilical vein endothelial cells (HUVECs) in order to detect possible district-specific differences. After isolation, the endothelium was cultured and stimulated with γ-interferon (IFN), tumour necrosis factor alpha (TNF-α) and LPS. The results showed that brain endothelium, in our experimental conditions, does not produce interleukin (IL)-10 and produces lower amounts of IL-1β and soluble intercellular adhesion molecule-1 (sICAM-1) than HUVECs do; no differences were detected in soluble vascular cell adhesion molecule-1 (sVCAM-1) production. MCP-1 mRNA was detected both without and after stimulation with TNF-α and γ-IFN in HUVECs and MS human brain endothelial cells (HBECs), while in non-MS-HBECs it was found only after γ-IFN stimulation.",
keywords = "Brain endothelium, IL-1 beta, IL-10, MCP-1",
author = "S. Frigerio and M. Gelati and E. Ciusani and E. Corsini and A. Dufour and G. Massa and A. Salmaggi",
year = "1998",
doi = "10.1007/s004150050275",
language = "English",
volume = "245",
pages = "727--730",
journal = "Journal of Neurology",
issn = "0340-5354",
publisher = "Dr. Dietrich Steinkopff Verlag GmbH and Co. KG",
number = "11",

}

TY - JOUR

T1 - Immunocompetence of human microvascular brain endothelial cells

T2 - Cytokine regulation of IL-1β, MCP-1, IL-10, sICAM-1 and sVCAM-1

AU - Frigerio, S.

AU - Gelati, M.

AU - Ciusani, E.

AU - Corsini, E.

AU - Dufour, A.

AU - Massa, G.

AU - Salmaggi, A.

PY - 1998

Y1 - 1998

N2 - Endothelia from the brains of four patients undergoing neurosurgery, including one multiple sclerosis (MS) patient, were studied in vitro to determine cytokine and chemokine production; the release of soluble adhesion molecules was also investigated. The same procedure was repeated on human umbilical vein endothelial cells (HUVECs) in order to detect possible district-specific differences. After isolation, the endothelium was cultured and stimulated with γ-interferon (IFN), tumour necrosis factor alpha (TNF-α) and LPS. The results showed that brain endothelium, in our experimental conditions, does not produce interleukin (IL)-10 and produces lower amounts of IL-1β and soluble intercellular adhesion molecule-1 (sICAM-1) than HUVECs do; no differences were detected in soluble vascular cell adhesion molecule-1 (sVCAM-1) production. MCP-1 mRNA was detected both without and after stimulation with TNF-α and γ-IFN in HUVECs and MS human brain endothelial cells (HBECs), while in non-MS-HBECs it was found only after γ-IFN stimulation.

AB - Endothelia from the brains of four patients undergoing neurosurgery, including one multiple sclerosis (MS) patient, were studied in vitro to determine cytokine and chemokine production; the release of soluble adhesion molecules was also investigated. The same procedure was repeated on human umbilical vein endothelial cells (HUVECs) in order to detect possible district-specific differences. After isolation, the endothelium was cultured and stimulated with γ-interferon (IFN), tumour necrosis factor alpha (TNF-α) and LPS. The results showed that brain endothelium, in our experimental conditions, does not produce interleukin (IL)-10 and produces lower amounts of IL-1β and soluble intercellular adhesion molecule-1 (sICAM-1) than HUVECs do; no differences were detected in soluble vascular cell adhesion molecule-1 (sVCAM-1) production. MCP-1 mRNA was detected both without and after stimulation with TNF-α and γ-IFN in HUVECs and MS human brain endothelial cells (HBECs), while in non-MS-HBECs it was found only after γ-IFN stimulation.

KW - Brain endothelium

KW - IL-1 beta

KW - IL-10

KW - MCP-1

UR - http://www.scopus.com/inward/record.url?scp=0031720461&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031720461&partnerID=8YFLogxK

U2 - 10.1007/s004150050275

DO - 10.1007/s004150050275

M3 - Article

C2 - 9808241

AN - SCOPUS:0031720461

VL - 245

SP - 727

EP - 730

JO - Journal of Neurology

JF - Journal of Neurology

SN - 0340-5354

IS - 11

ER -