Immunocompetence of human microvascular brain endothelial cells: Cytokine regulation of IL-1β, MCP-1, IL-10, sICAM-1 and sVCAM-1

S. Frigerio, M. Gelati, E. Ciusani, E. Corsini, A. Dufour, G. Massa, A. Salmaggi

Research output: Contribution to journalArticle

Abstract

Endothelia from the brains of four patients undergoing neurosurgery, including one multiple sclerosis (MS) patient, were studied in vitro to determine cytokine and chemokine production; the release of soluble adhesion molecules was also investigated. The same procedure was repeated on human umbilical vein endothelial cells (HUVECs) in order to detect possible district-specific differences. After isolation, the endothelium was cultured and stimulated with γ-interferon (IFN), tumour necrosis factor alpha (TNF-α) and LPS. The results showed that brain endothelium, in our experimental conditions, does not produce interleukin (IL)-10 and produces lower amounts of IL-1β and soluble intercellular adhesion molecule-1 (sICAM-1) than HUVECs do; no differences were detected in soluble vascular cell adhesion molecule-1 (sVCAM-1) production. MCP-1 mRNA was detected both without and after stimulation with TNF-α and γ-IFN in HUVECs and MS human brain endothelial cells (HBECs), while in non-MS-HBECs it was found only after γ-IFN stimulation.

Original languageEnglish
Pages (from-to)727-730
Number of pages4
JournalJournal of Neurology
Volume245
Issue number11
DOIs
Publication statusPublished - 1998

Keywords

  • Brain endothelium
  • IL-1 beta
  • IL-10
  • MCP-1

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

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