Immunocytochemical analysis of contact Lens surface deposits in transmission electron microscopy

Piera Versura; Maria Cristina Maltarello; Roberto Caramazza; Renzo Laschi

doi:10.3109/02713688809047033

Immunocytochemical analysis of contact Lens surface deposits in transmission electron microscopy

Piera Versura, Maria Cristina Maltarello, Roberto Caramazza, Renzo Laschi

Istituti Ortopedici Rizzoli

Research output: Contribution to journal › Article › peer-review

Abstract

We studied 8 soft contact lenses from asymptomatic wearers by means of an immuno-cytochemical method, in transmission electron microscopy. In particular, the presence of IgA, IgG, IgE, C_1q complement fraction within the surface deposits was analyzed. All the lenses were found positive for the immunoglobulins and the C_1q, being the tarsal side more heavily coated than the corneal one. IgA was the predominant Ig, followed by IgG, IgE, and C_1q in this descending order. New, never worn lenses were found completely negative for any of the proteins under investigation. We conclude that the Igs come from the tear fluid and speculate about the C_1q as a possible sign of involvement of the host immuno-defense mechanism against the prosthesis.

Original language	English
Pages (from-to)	277-286
Number of pages	10
Journal	Current Eye Research
Volume	7
Issue number	3
DOIs	https://doi.org/10.3109/02713688809047033
Publication status	Published - 1988

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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abstract = "We studied 8 soft contact lenses from asymptomatic wearers by means of an immuno-cytochemical method, in transmission electron microscopy. In particular, the presence of IgA, IgG, IgE, C1q complement fraction within the surface deposits was analyzed. All the lenses were found positive for the immunoglobulins and the C1q, being the tarsal side more heavily coated than the corneal one. IgA was the predominant Ig, followed by IgG, IgE, and C1q in this descending order. New, never worn lenses were found completely negative for any of the proteins under investigation. We conclude that the Igs come from the tear fluid and speculate about the C1q as a possible sign of involvement of the host immuno-defense mechanism against the prosthesis.",

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AU - Versura, Piera

AU - Maltarello, Maria Cristina

AU - Caramazza, Roberto

AU - Laschi, Renzo

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AB - We studied 8 soft contact lenses from asymptomatic wearers by means of an immuno-cytochemical method, in transmission electron microscopy. In particular, the presence of IgA, IgG, IgE, C1q complement fraction within the surface deposits was analyzed. All the lenses were found positive for the immunoglobulins and the C1q, being the tarsal side more heavily coated than the corneal one. IgA was the predominant Ig, followed by IgG, IgE, and C1q in this descending order. New, never worn lenses were found completely negative for any of the proteins under investigation. We conclude that the Igs come from the tear fluid and speculate about the C1q as a possible sign of involvement of the host immuno-defense mechanism against the prosthesis.

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