Immunocytochemical analysis of contact Lens surface deposits in transmission electron microscopy

Piera Versura, Maria Cristina Maltarello, Roberto Caramazza, Renzo Laschi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We studied 8 soft contact lenses from asymptomatic wearers by means of an immuno-cytochemical method, in transmission electron microscopy. In particular, the presence of IgA, IgG, IgE, C1q complement fraction within the surface deposits was analyzed. All the lenses were found positive for the immunoglobulins and the C1q, being the tarsal side more heavily coated than the corneal one. IgA was the predominant Ig, followed by IgG, IgE, and C1q in this descending order. New, never worn lenses were found completely negative for any of the proteins under investigation. We conclude that the Igs come from the tear fluid and speculate about the C1q as a possible sign of involvement of the host immuno-defense mechanism against the prosthesis.

Original languageEnglish
Pages (from-to)277-286
Number of pages10
JournalCurrent Eye Research
Volume7
Issue number3
DOIs
Publication statusPublished - 1988

Fingerprint

Contact Lenses
Transmission Electron Microscopy
Immunoglobulin A
Immunoglobulin E
Lenses
Immunoglobulin G
Complement C1q
Hydrophilic Contact Lens
Tears
Prostheses and Implants
Immunoglobulins
Proteins

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Immunocytochemical analysis of contact Lens surface deposits in transmission electron microscopy. / Versura, Piera; Maltarello, Maria Cristina; Caramazza, Roberto; Laschi, Renzo.

In: Current Eye Research, Vol. 7, No. 3, 1988, p. 277-286.

Research output: Contribution to journalArticle

Versura, Piera ; Maltarello, Maria Cristina ; Caramazza, Roberto ; Laschi, Renzo. / Immunocytochemical analysis of contact Lens surface deposits in transmission electron microscopy. In: Current Eye Research. 1988 ; Vol. 7, No. 3. pp. 277-286.
@article{3624eef699e348d9a44466b01cd3791f,
title = "Immunocytochemical analysis of contact Lens surface deposits in transmission electron microscopy",
abstract = "We studied 8 soft contact lenses from asymptomatic wearers by means of an immuno-cytochemical method, in transmission electron microscopy. In particular, the presence of IgA, IgG, IgE, C1q complement fraction within the surface deposits was analyzed. All the lenses were found positive for the immunoglobulins and the C1q, being the tarsal side more heavily coated than the corneal one. IgA was the predominant Ig, followed by IgG, IgE, and C1q in this descending order. New, never worn lenses were found completely negative for any of the proteins under investigation. We conclude that the Igs come from the tear fluid and speculate about the C1q as a possible sign of involvement of the host immuno-defense mechanism against the prosthesis.",
author = "Piera Versura and Maltarello, {Maria Cristina} and Roberto Caramazza and Renzo Laschi",
year = "1988",
doi = "10.3109/02713688809047033",
language = "English",
volume = "7",
pages = "277--286",
journal = "Current Eye Research",
issn = "0271-3683",
publisher = "Informa Healthcare",
number = "3",

}

TY - JOUR

T1 - Immunocytochemical analysis of contact Lens surface deposits in transmission electron microscopy

AU - Versura, Piera

AU - Maltarello, Maria Cristina

AU - Caramazza, Roberto

AU - Laschi, Renzo

PY - 1988

Y1 - 1988

N2 - We studied 8 soft contact lenses from asymptomatic wearers by means of an immuno-cytochemical method, in transmission electron microscopy. In particular, the presence of IgA, IgG, IgE, C1q complement fraction within the surface deposits was analyzed. All the lenses were found positive for the immunoglobulins and the C1q, being the tarsal side more heavily coated than the corneal one. IgA was the predominant Ig, followed by IgG, IgE, and C1q in this descending order. New, never worn lenses were found completely negative for any of the proteins under investigation. We conclude that the Igs come from the tear fluid and speculate about the C1q as a possible sign of involvement of the host immuno-defense mechanism against the prosthesis.

AB - We studied 8 soft contact lenses from asymptomatic wearers by means of an immuno-cytochemical method, in transmission electron microscopy. In particular, the presence of IgA, IgG, IgE, C1q complement fraction within the surface deposits was analyzed. All the lenses were found positive for the immunoglobulins and the C1q, being the tarsal side more heavily coated than the corneal one. IgA was the predominant Ig, followed by IgG, IgE, and C1q in this descending order. New, never worn lenses were found completely negative for any of the proteins under investigation. We conclude that the Igs come from the tear fluid and speculate about the C1q as a possible sign of involvement of the host immuno-defense mechanism against the prosthesis.

UR - http://www.scopus.com/inward/record.url?scp=0023856586&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023856586&partnerID=8YFLogxK

U2 - 10.3109/02713688809047033

DO - 10.3109/02713688809047033

M3 - Article

C2 - 3258806

AN - SCOPUS:0023856586

VL - 7

SP - 277

EP - 286

JO - Current Eye Research

JF - Current Eye Research

SN - 0271-3683

IS - 3

ER -