Abstract
We studied 8 soft contact lenses from asymptomatic wearers by means of an immuno-cytochemical method, in transmission electron microscopy. In particular, the presence of IgA, IgG, IgE, C1q complement fraction within the surface deposits was analyzed. All the lenses were found positive for the immunoglobulins and the C1q, being the tarsal side more heavily coated than the corneal one. IgA was the predominant Ig, followed by IgG, IgE, and C1q in this descending order. New, never worn lenses were found completely negative for any of the proteins under investigation. We conclude that the Igs come from the tear fluid and speculate about the C1q as a possible sign of involvement of the host immuno-defense mechanism against the prosthesis.
| Original language | English |
|---|---|
| Pages (from-to) | 277-286 |
| Number of pages | 10 |
| Journal | Current Eye Research |
| Volume | 7 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1988 |
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ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience
Cite this
Immunocytochemical analysis of contact Lens surface deposits in transmission electron microscopy. / Versura, Piera; Maltarello, Maria Cristina; Caramazza, Roberto; Laschi, Renzo.
In: Current Eye Research, Vol. 7, No. 3, 1988, p. 277-286.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunocytochemical analysis of contact Lens surface deposits in transmission electron microscopy
AU - Versura, Piera
AU - Maltarello, Maria Cristina
AU - Caramazza, Roberto
AU - Laschi, Renzo
PY - 1988
Y1 - 1988
N2 - We studied 8 soft contact lenses from asymptomatic wearers by means of an immuno-cytochemical method, in transmission electron microscopy. In particular, the presence of IgA, IgG, IgE, C1q complement fraction within the surface deposits was analyzed. All the lenses were found positive for the immunoglobulins and the C1q, being the tarsal side more heavily coated than the corneal one. IgA was the predominant Ig, followed by IgG, IgE, and C1q in this descending order. New, never worn lenses were found completely negative for any of the proteins under investigation. We conclude that the Igs come from the tear fluid and speculate about the C1q as a possible sign of involvement of the host immuno-defense mechanism against the prosthesis.
AB - We studied 8 soft contact lenses from asymptomatic wearers by means of an immuno-cytochemical method, in transmission electron microscopy. In particular, the presence of IgA, IgG, IgE, C1q complement fraction within the surface deposits was analyzed. All the lenses were found positive for the immunoglobulins and the C1q, being the tarsal side more heavily coated than the corneal one. IgA was the predominant Ig, followed by IgG, IgE, and C1q in this descending order. New, never worn lenses were found completely negative for any of the proteins under investigation. We conclude that the Igs come from the tear fluid and speculate about the C1q as a possible sign of involvement of the host immuno-defense mechanism against the prosthesis.
UR - http://www.scopus.com/inward/record.url?scp=0023856586&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023856586&partnerID=8YFLogxK
U2 - 10.3109/02713688809047033
DO - 10.3109/02713688809047033
M3 - Article
C2 - 3258806
AN - SCOPUS:0023856586
VL - 7
SP - 277
EP - 286
JO - Current Eye Research
JF - Current Eye Research
SN - 0271-3683
IS - 3
ER -