Immunocytochemical assay of estrogen and progesterone receptors in fine needle aspirates from breast cancer patients

R. Nizzoli, C. Bozzetti, L. Savoldi, L. Manotti, N. Naldi, R. Camisa, A. P. Soresi, A. Guazzi, G. Cocconi

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Estrogen receptors (ERs) and progesterone receptors (PRs) were determined by an immunocytochemical assay (ICA) on fine needle aspirates (FNAs) from patients with primary, recurrent and metastatic mammary carcinoma, and the results were compared to those with the biochemical dextran-coated charcoal (DCC) method performed on the surgical sample in order to compare the two methods. The aspirates were suspended in a buffered saline solution, cytocentrifuged onto glass slides and immunocytochemically stained according to the protocol of commercial kits employing monoclonal antibodies specific for ER and PR. Immunocytochemical staining of malignant cells was evaluated on the basis of the percentage of stained cells; 10% staining was taken as the cutoff value. Fine needle aspiration biopsies (FNABs) from 107 breast carcinomas were analyzed immunocytochemically for ER and 31 of them for PR, also. The overall concordance between ICA and DCC was 88% for ER and 87% for PR. The sensitivity, specificity, and positive and negative predictive value of ICA on FNAs as compared to conventional DCC were 87%, 90%, 97% and 63%, respectively, for ER and 85%, 100%, 100% and 56% for PR. These findings suggest that estrogen immunocytochemical assays and progesterone immunocytochemical assays on FNAs in breast cancer patients are reliable techniques for evaluating receptor status and can be useful in assessing ER and PR whenever surgical biopsy is not indicated and when information about ER and PR status is required at the time of the clinical diagnosis.

Original languageEnglish
Pages (from-to)933-938
Number of pages6
JournalActa Cytologica
Volume38
Issue number6
Publication statusPublished - 1994

Fingerprint

Progesterone Receptors
Estrogen Receptors
Needles
Breast Neoplasms
Charcoal
Dextrans
Staining and Labeling
Fine Needle Biopsy
Sodium Chloride
Glass
Progesterone
Estrogens
Monoclonal Antibodies
Biopsy
Sensitivity and Specificity

Keywords

  • aspiration biopsy
  • breast neoplasms
  • estrogen receptors
  • immunocytochemistry
  • progesterone receptors

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology
  • Histology

Cite this

Nizzoli, R., Bozzetti, C., Savoldi, L., Manotti, L., Naldi, N., Camisa, R., ... Cocconi, G. (1994). Immunocytochemical assay of estrogen and progesterone receptors in fine needle aspirates from breast cancer patients. Acta Cytologica, 38(6), 933-938.

Immunocytochemical assay of estrogen and progesterone receptors in fine needle aspirates from breast cancer patients. / Nizzoli, R.; Bozzetti, C.; Savoldi, L.; Manotti, L.; Naldi, N.; Camisa, R.; Soresi, A. P.; Guazzi, A.; Cocconi, G.

In: Acta Cytologica, Vol. 38, No. 6, 1994, p. 933-938.

Research output: Contribution to journalArticle

Nizzoli, R, Bozzetti, C, Savoldi, L, Manotti, L, Naldi, N, Camisa, R, Soresi, AP, Guazzi, A & Cocconi, G 1994, 'Immunocytochemical assay of estrogen and progesterone receptors in fine needle aspirates from breast cancer patients', Acta Cytologica, vol. 38, no. 6, pp. 933-938.
Nizzoli R, Bozzetti C, Savoldi L, Manotti L, Naldi N, Camisa R et al. Immunocytochemical assay of estrogen and progesterone receptors in fine needle aspirates from breast cancer patients. Acta Cytologica. 1994;38(6):933-938.
Nizzoli, R. ; Bozzetti, C. ; Savoldi, L. ; Manotti, L. ; Naldi, N. ; Camisa, R. ; Soresi, A. P. ; Guazzi, A. ; Cocconi, G. / Immunocytochemical assay of estrogen and progesterone receptors in fine needle aspirates from breast cancer patients. In: Acta Cytologica. 1994 ; Vol. 38, No. 6. pp. 933-938.
@article{146e805168d44c64b7766e417f574c00,
title = "Immunocytochemical assay of estrogen and progesterone receptors in fine needle aspirates from breast cancer patients",
abstract = "Estrogen receptors (ERs) and progesterone receptors (PRs) were determined by an immunocytochemical assay (ICA) on fine needle aspirates (FNAs) from patients with primary, recurrent and metastatic mammary carcinoma, and the results were compared to those with the biochemical dextran-coated charcoal (DCC) method performed on the surgical sample in order to compare the two methods. The aspirates were suspended in a buffered saline solution, cytocentrifuged onto glass slides and immunocytochemically stained according to the protocol of commercial kits employing monoclonal antibodies specific for ER and PR. Immunocytochemical staining of malignant cells was evaluated on the basis of the percentage of stained cells; 10{\%} staining was taken as the cutoff value. Fine needle aspiration biopsies (FNABs) from 107 breast carcinomas were analyzed immunocytochemically for ER and 31 of them for PR, also. The overall concordance between ICA and DCC was 88{\%} for ER and 87{\%} for PR. The sensitivity, specificity, and positive and negative predictive value of ICA on FNAs as compared to conventional DCC were 87{\%}, 90{\%}, 97{\%} and 63{\%}, respectively, for ER and 85{\%}, 100{\%}, 100{\%} and 56{\%} for PR. These findings suggest that estrogen immunocytochemical assays and progesterone immunocytochemical assays on FNAs in breast cancer patients are reliable techniques for evaluating receptor status and can be useful in assessing ER and PR whenever surgical biopsy is not indicated and when information about ER and PR status is required at the time of the clinical diagnosis.",
keywords = "aspiration biopsy, breast neoplasms, estrogen receptors, immunocytochemistry, progesterone receptors",
author = "R. Nizzoli and C. Bozzetti and L. Savoldi and L. Manotti and N. Naldi and R. Camisa and Soresi, {A. P.} and A. Guazzi and G. Cocconi",
year = "1994",
language = "English",
volume = "38",
pages = "933--938",
journal = "Acta Cytologica",
issn = "0001-5547",
publisher = "Science Printers and Publishers Inc.",
number = "6",

}

TY - JOUR

T1 - Immunocytochemical assay of estrogen and progesterone receptors in fine needle aspirates from breast cancer patients

AU - Nizzoli, R.

AU - Bozzetti, C.

AU - Savoldi, L.

AU - Manotti, L.

AU - Naldi, N.

AU - Camisa, R.

AU - Soresi, A. P.

AU - Guazzi, A.

AU - Cocconi, G.

PY - 1994

Y1 - 1994

N2 - Estrogen receptors (ERs) and progesterone receptors (PRs) were determined by an immunocytochemical assay (ICA) on fine needle aspirates (FNAs) from patients with primary, recurrent and metastatic mammary carcinoma, and the results were compared to those with the biochemical dextran-coated charcoal (DCC) method performed on the surgical sample in order to compare the two methods. The aspirates were suspended in a buffered saline solution, cytocentrifuged onto glass slides and immunocytochemically stained according to the protocol of commercial kits employing monoclonal antibodies specific for ER and PR. Immunocytochemical staining of malignant cells was evaluated on the basis of the percentage of stained cells; 10% staining was taken as the cutoff value. Fine needle aspiration biopsies (FNABs) from 107 breast carcinomas were analyzed immunocytochemically for ER and 31 of them for PR, also. The overall concordance between ICA and DCC was 88% for ER and 87% for PR. The sensitivity, specificity, and positive and negative predictive value of ICA on FNAs as compared to conventional DCC were 87%, 90%, 97% and 63%, respectively, for ER and 85%, 100%, 100% and 56% for PR. These findings suggest that estrogen immunocytochemical assays and progesterone immunocytochemical assays on FNAs in breast cancer patients are reliable techniques for evaluating receptor status and can be useful in assessing ER and PR whenever surgical biopsy is not indicated and when information about ER and PR status is required at the time of the clinical diagnosis.

AB - Estrogen receptors (ERs) and progesterone receptors (PRs) were determined by an immunocytochemical assay (ICA) on fine needle aspirates (FNAs) from patients with primary, recurrent and metastatic mammary carcinoma, and the results were compared to those with the biochemical dextran-coated charcoal (DCC) method performed on the surgical sample in order to compare the two methods. The aspirates were suspended in a buffered saline solution, cytocentrifuged onto glass slides and immunocytochemically stained according to the protocol of commercial kits employing monoclonal antibodies specific for ER and PR. Immunocytochemical staining of malignant cells was evaluated on the basis of the percentage of stained cells; 10% staining was taken as the cutoff value. Fine needle aspiration biopsies (FNABs) from 107 breast carcinomas were analyzed immunocytochemically for ER and 31 of them for PR, also. The overall concordance between ICA and DCC was 88% for ER and 87% for PR. The sensitivity, specificity, and positive and negative predictive value of ICA on FNAs as compared to conventional DCC were 87%, 90%, 97% and 63%, respectively, for ER and 85%, 100%, 100% and 56% for PR. These findings suggest that estrogen immunocytochemical assays and progesterone immunocytochemical assays on FNAs in breast cancer patients are reliable techniques for evaluating receptor status and can be useful in assessing ER and PR whenever surgical biopsy is not indicated and when information about ER and PR status is required at the time of the clinical diagnosis.

KW - aspiration biopsy

KW - breast neoplasms

KW - estrogen receptors

KW - immunocytochemistry

KW - progesterone receptors

UR - http://www.scopus.com/inward/record.url?scp=0028560361&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028560361&partnerID=8YFLogxK

M3 - Article

C2 - 7992582

AN - SCOPUS:0028560361

VL - 38

SP - 933

EP - 938

JO - Acta Cytologica

JF - Acta Cytologica

SN - 0001-5547

IS - 6

ER -