Immunocytochemical distribution of extracellular matrix receptors in human osteoclasts: A β3 integrin is colocalized with vinculin and talin in the podosomes of osteoclastoma giant cells

Alberta Zambonin-Zallone, Anna Teti, M. Grano, Alessandro Rubinacci, Marzia Abbadini, Mirella Gaboli, Pier Carlo Marchisio

Research output: Contribution to journalArticle

Abstract

Human osteoclasts (OCLs) obtained from cell suspensions of surgically excised giant cell bone tumors (osteoclastomas) were attached to glass coverslips and analyzed by immunofluorescence with antibodies to integrins and cytoskeletal proteins. It was found that in OCLs (i) podosomes, identified by their F-actin core and by interference reflection microscopy, were predominantly found in a peripheral belt as described in avian OCLs; (ii) each F-actin core was surrounded by a ring of vinculin and talin; (iii) β1 integrin was diffuse in the ventral membrane; (iv) β3 integrin was distributed in intensely fluorescent rings surrounding F-actin cores; (v) β2 integrin was absent; (vi) β4 integrin was absent. The macrophages detected in the same coverslips displayed podosomes containing β2 but not β3, fibroblasts showed adhesion plaques positive for β1 and β3 but not for β2, and platelets were intensely positive for β3. These results indicate that OCLs produce an integrin complex that is absent in the monocyte-macrophage lineage.

Original languageEnglish
Pages (from-to)645-652
Number of pages8
JournalExperimental Cell Research
Volume182
Issue number2
DOIs
Publication statusPublished - 1989

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Talin
Vinculin
Osteoclasts
Giant Cells
Integrins
Actins
Macrophages
Interference Microscopy
Giant Cell Tumor of Bone
Focal Adhesions
Cytoskeletal Proteins
Glass
Fluorescent Antibody Technique
extracellular matrix receptor
Podosomes
Monocytes
Suspensions
Blood Platelets
Fibroblasts
Membranes

ASJC Scopus subject areas

  • Cell Biology

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Immunocytochemical distribution of extracellular matrix receptors in human osteoclasts : A β3 integrin is colocalized with vinculin and talin in the podosomes of osteoclastoma giant cells. / Zambonin-Zallone, Alberta; Teti, Anna; Grano, M.; Rubinacci, Alessandro; Abbadini, Marzia; Gaboli, Mirella; Marchisio, Pier Carlo.

In: Experimental Cell Research, Vol. 182, No. 2, 1989, p. 645-652.

Research output: Contribution to journalArticle

Zambonin-Zallone, Alberta ; Teti, Anna ; Grano, M. ; Rubinacci, Alessandro ; Abbadini, Marzia ; Gaboli, Mirella ; Marchisio, Pier Carlo. / Immunocytochemical distribution of extracellular matrix receptors in human osteoclasts : A β3 integrin is colocalized with vinculin and talin in the podosomes of osteoclastoma giant cells. In: Experimental Cell Research. 1989 ; Vol. 182, No. 2. pp. 645-652.
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