Human osteoclasts (OCLs) obtained from cell suspensions of surgically excised giant cell bone tumors (osteoclastomas) were attached to glass coverslips and analyzed by immunofluorescence with antibodies to integrins and cytoskeletal proteins. It was found that in OCLs (i) podosomes, identified by their F-actin core and by interference reflection microscopy, were predominantly found in a peripheral belt as described in avian OCLs; (ii) each F-actin core was surrounded by a ring of vinculin and talin; (iii) β1 integrin was diffuse in the ventral membrane; (iv) β3 integrin was distributed in intensely fluorescent rings surrounding F-actin cores; (v) β2 integrin was absent; (vi) β4 integrin was absent. The macrophages detected in the same coverslips displayed podosomes containing β2 but not β3, fibroblasts showed adhesion plaques positive for β1 and β3 but not for β2, and platelets were intensely positive for β3. These results indicate that OCLs produce an integrin complex that is absent in the monocyte-macrophage lineage.
ASJC Scopus subject areas
- Cell Biology