Abstract
Human osteoclasts (OCLs) obtained from cell suspensions of surgically excised giant cell bone tumors (osteoclastomas) were attached to glass coverslips and analyzed by immunofluorescence with antibodies to integrins and cytoskeletal proteins. It was found that in OCLs (i) podosomes, identified by their F-actin core and by interference reflection microscopy, were predominantly found in a peripheral belt as described in avian OCLs; (ii) each F-actin core was surrounded by a ring of vinculin and talin; (iii) β1 integrin was diffuse in the ventral membrane; (iv) β3 integrin was distributed in intensely fluorescent rings surrounding F-actin cores; (v) β2 integrin was absent; (vi) β4 integrin was absent. The macrophages detected in the same coverslips displayed podosomes containing β2 but not β3, fibroblasts showed adhesion plaques positive for β1 and β3 but not for β2, and platelets were intensely positive for β3. These results indicate that OCLs produce an integrin complex that is absent in the monocyte-macrophage lineage.
| Original language | English |
|---|---|
| Pages (from-to) | 645-652 |
| Number of pages | 8 |
| Journal | Experimental Cell Research |
| Volume | 182 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1989 |
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Immunocytochemical distribution of extracellular matrix receptors in human osteoclasts : A β3 integrin is colocalized with vinculin and talin in the podosomes of osteoclastoma giant cells. / Zambonin-Zallone, Alberta; Teti, Anna; Grano, M.; Rubinacci, Alessandro; Abbadini, Marzia; Gaboli, Mirella; Marchisio, Pier Carlo.
In: Experimental Cell Research, Vol. 182, No. 2, 1989, p. 645-652.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunocytochemical distribution of extracellular matrix receptors in human osteoclasts
T2 - A β3 integrin is colocalized with vinculin and talin in the podosomes of osteoclastoma giant cells
AU - Zambonin-Zallone, Alberta
AU - Teti, Anna
AU - Grano, M.
AU - Rubinacci, Alessandro
AU - Abbadini, Marzia
AU - Gaboli, Mirella
AU - Marchisio, Pier Carlo
PY - 1989
Y1 - 1989
N2 - Human osteoclasts (OCLs) obtained from cell suspensions of surgically excised giant cell bone tumors (osteoclastomas) were attached to glass coverslips and analyzed by immunofluorescence with antibodies to integrins and cytoskeletal proteins. It was found that in OCLs (i) podosomes, identified by their F-actin core and by interference reflection microscopy, were predominantly found in a peripheral belt as described in avian OCLs; (ii) each F-actin core was surrounded by a ring of vinculin and talin; (iii) β1 integrin was diffuse in the ventral membrane; (iv) β3 integrin was distributed in intensely fluorescent rings surrounding F-actin cores; (v) β2 integrin was absent; (vi) β4 integrin was absent. The macrophages detected in the same coverslips displayed podosomes containing β2 but not β3, fibroblasts showed adhesion plaques positive for β1 and β3 but not for β2, and platelets were intensely positive for β3. These results indicate that OCLs produce an integrin complex that is absent in the monocyte-macrophage lineage.
AB - Human osteoclasts (OCLs) obtained from cell suspensions of surgically excised giant cell bone tumors (osteoclastomas) were attached to glass coverslips and analyzed by immunofluorescence with antibodies to integrins and cytoskeletal proteins. It was found that in OCLs (i) podosomes, identified by their F-actin core and by interference reflection microscopy, were predominantly found in a peripheral belt as described in avian OCLs; (ii) each F-actin core was surrounded by a ring of vinculin and talin; (iii) β1 integrin was diffuse in the ventral membrane; (iv) β3 integrin was distributed in intensely fluorescent rings surrounding F-actin cores; (v) β2 integrin was absent; (vi) β4 integrin was absent. The macrophages detected in the same coverslips displayed podosomes containing β2 but not β3, fibroblasts showed adhesion plaques positive for β1 and β3 but not for β2, and platelets were intensely positive for β3. These results indicate that OCLs produce an integrin complex that is absent in the monocyte-macrophage lineage.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024324937&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(89)90266-8
DO - 10.1016/0014-4827(89)90266-8
M3 - Article
C2 - 2542071
AN - SCOPUS:0024324937
VL - 182
SP - 645
EP - 652
JO - Experimental Cell Research
JF - Experimental Cell Research
SN - 0014-4827
IS - 2
ER -